丙泊酚对胰腺癌Panc1细胞株典型细胞生物学行为的影响

Effect of Propofol on biological behaviors of Pancreatic tumor (Panc1) cells

目的观察丙泊酚对胰腺癌细胞粘附能力、迁移能力及细胞活性的影响。方法将胰腺癌Panc1细胞株与含有不同浓度丙泊酚的培养基共培养,分为对照组(C组)、丙泊酚5μg/ml组(P5组)和丙泊酚10μg/ml组(P10组)。C组直接将Panc1细胞株接种在培养基中,不加任何药物;P5组培养基为5μg/ml丙泊酚+Panc1细胞株;P10组培养基为10μg/ml丙泊酚+Panc1细胞株。分别采用细胞粘附实验、细胞划痕实验和四甲基偶氮唑盐(MTT)法,观察不同浓度的丙泊酚对胰腺癌细胞粘附作用、迁移率和细胞活性的影响。结果在荧光显微镜视野中C组粘附的Panc1细胞个数最多,P5组次之,P10组最少。P5组和P10组细胞数明显少于C组(P<0.05),P10组细胞数明显少于P5组(P<0.05)。8h的P5组和P10组细胞迁移速率明显慢于C组(P<0.05),且P10组细胞迁移速率明显慢于P5组(P<0.05)。P5组和P10组活性细胞百分比明显低于C组(P<0.05),P10组活性细胞百分比明显低于P5组(P<0.05)。结论丙泊酚能够有效抑制胰腺癌细胞粘附能力、迁移能力及细胞活性;且10μg/ml丙泊酚抑制效果明显优于5μg/ml丙泊酚。

Objective To observe the effect of propofol on adhesion ability, migration ability and viability of pancreatic tumor(Panc1) cells. Methods Panc1 cells were cultured with different concentrations of propofol and divided into three groups: Control group(group C), propofol 5 μg/ml group(group P5) and Propofol 10 μg/ml group(group P10). The dose of propofol was 0 μg/ml in group C, 5 μg/ml in group P5 and 10 μg/ml in group P10. Adhesion ability, migration ability and viability of Panc1 cells were tested by adhesion assay, wound healing and MTT assay. Results In the field of microscope, the number of adhered Panc1 cells could be observed. Compared with group C, the number of cells in group P5 or group P10 was significantly reduced(P<0.05), and the number of cells in group P10 was significantly reduced compared with group P5(P<0.05). Compared with group C, the migration rate of group P5 or group P10 was significantly slower at 8 h(P<0.05), and the migration rate of group P10 was significantly slower than that of group P5(P<0.05). Moreover, the cell activity of group P5 or group P10 was significantly lower than that of group C(P<0.05), and the cell activity of group P10 was significantly lower than that of group P5(P<0.05). Conclusion Propofol could effectively inhibit the adhesion ability, migration ability and viability of pancreatic tumor cells. The inhibitory effect of 10 μg/ml propofol was better than 5 μg/ml propofol.