金纳米膜顶空固相萃取-表面增强拉曼光谱法快速检测挥发性多环芳烃

Rapid detection of volatile polycyclic aromatic hydrocarbons using gold nanofilms by coupling headspace solid phase solid phase extraction with surface-enhanced Raman spectroscopy

采用氧化还原反应将金纳米颗粒沉淀在不锈钢片表面,形成纳米膜制备表面增强拉曼光谱(SERS)基底;以多环芳烃(芘、萘和间三联苯)为探针,结合顶空固相萃取法,考察了金纳米膜用作SERS基底的检测性能。结果表明:芘、萘和间三联苯皆可在室温下检测出拉曼光谱信号,在40℃,55℃和70℃进行顶空固相萃取后,可分别获得芘、萘和间三联苯更显著的SERS信号;这3种多环芳烃仅需要顶空固相萃取5 min,即可检测出相应的拉曼光谱信号,可用于快速检测;芘、萘和间三联苯各自在40℃,55℃,70℃顶空固相萃取后,对应的检测限(LOD)分别为10,10,50 ng/L,相关系数分别为0.9239,0.9545,0.9559。在以赣江水和自来水为载体的水样中,可检测出质量浓度为1.0 ng/L的芘、萘和间三联苯。方法可用于食品安全和环境检测。

The gold nanoparticles were precipitated on the surface of the stainless steel sheet by redox reaction to form a surface-enhanced Raman spectroscopy(SERS)substrate.The detection properties of gold nanofilms as SERS substrates were investigated by using polycyclic aromatic hydrocarbons(pyrene,naphthalene and m-terphenyl)as probes and headspace solid phase extraction.The results showed that pyrene,naphthalene and m-terphenyl could be detected at room temperature;after headspace solid phase extraction at 40℃,55℃ and 70℃,stronger SERS signals of pyrene,naphthalene and m-terphenyl could be obtained respectively.These three polycyclic aromatic hydrocarbons required only headspace solid phase extraction for 5 min to detect the corresponding Raman spectral signal,which could be used for rapid detection.The limits of detection(LOD)of pyrene,naphthalene and m-terphenyl were 10,10,50 ng/L(70℃),respectively.The partial least squares(PLS)quantitative model showed that correlation coefficients were 0.9238,0.9018 and 0.9559,respectively.Pyrene,naphthalene and m-terphenyl from the Ganjiang River and tap water,could be detected at a concentration of 1.0 ng/L.The detection method has a good prospect in food safety and environmental detection.