miR-135a和miR-146a在阿尔茨海默病患者中的表达及诊断效能评价

Expression and diagnostic efficacy of miR-135a and miR-146a in patients with Alzheimer′s disease

目的探讨miR-135a和miR-146a在阿尔茨海默病(AD)患者中的表达及临床意义,为AD的诊断提供依据。方法选取2017年6月—2019年5月浙江省台州市第一人民医院(以下简称“我院”)神经内科住院的65例AD患者为AD组,另选取同期在我院体检的30名健康体检者作为对照组。采用实时荧光定量PCR检测miR-135a和miR-146a表达水平,受试者工作特征(ROC)曲线评价miR-135a和miR-146a对AD诊断效能。结果AD组miR-135a表达低于对照组,miR-146a表达高于对照组,差异均有统计学意义(均P<0.05)。miR-135a诊断AD的临界值为0.825,敏感度为0.923,特异性为0.700;miR-146a诊断AD的临界值为2.065,敏感度为0.877,特异性为0.900。结论AD患者中miR-135a表达下调,miR-146a表达上调,对AD有较高的诊断效能。

Objective To investigate the expression and clinical significance of miR-135a and miR-146a in patients with Alzheimer′s disease(AD),and to provide evidence for the diagnosis of AD.Methods Sixty-five patients with AD who admitted to Department of Neurology,the First People′s Hospital of Taizhou of Zhejiang Province(“our hospital”for short)from June 2017 to May 2019 were enrolled in AD group.In addition,30 healthy subjects who underwent physical examination in our hospital during the same period were selected as control group.The expression levels of miR-135a and miR-146a were detected by real-time fluorescent quantitative PCR and the diagnostic efficacy of miR-135a and miR-146a in AD were evaluated by receiver operating characteristic(ROC)curve.Results The expression of miR-135a in AD group was lower than that in control group,and the expression of miR-146a was higher than that in control group,with statistically significant differences(all P<0.05).The cut-off value of miR-135a in the diagnosis of AD was 0.825,the sensitivity was 0.923,and the specificity was 0.700.The cut-off value of miR-146a in the diagnosis of AD was 2.065,the sensitivity was 0.877,and the specificity was 0.900.Conclusion The expression of miR-135a is down-regulated,the expression of miR-146a is up-regulated in AD patients,which have a higher diagnostic efficacy for AD.