P16、Ki-67表达与直肠癌患者预后的关系

Relationship between the expression of P16 and Ki-67 and the prognosis of patients with rectal cancer

目的研究多肿瘤抑制基因P16、细胞增殖核抗原Ki-67表达与直肠癌患者预后的关系。方法选择2016年8月—2018年12月浙江省杭州市萧山区第一人民医院60例直肠癌患者术后的肿瘤及癌旁正常组织,以免疫组织法检测P16、Ki-67的表达情况,观察P16、Ki-67在不同年龄、病理类型、TNM分期、分化程度及淋巴结有无转移中的阳性率,比较P16、Ki-67阳性患者与阴性患者术后1年的生存情况,通过Spearman系数分析P16和Ki-67的相关性。结果直肠癌癌组织中P16及Ki-67的阳性率显著高于癌旁正常组织(P<0.05)。TNM分期Ⅲ期、高分化程度患者P16、Ki-67阳性率显著高于TNM分期Ⅱ期、低-中分化患者(P<0.05);有淋巴结转移患者Ki-67阳性率显著高于无淋巴结转移患者,有淋巴结转移患者P16阳性率显著高于无淋巴结转移患者(均P<0.05)。P16阴性患者生存率显著高于阳性患者(P<0.05)。Ki-67阳性患者1年生存率显著低于Ki-67阴性患者(P<0.05)。经相关性分析P16和Ki-67直肠癌组织中的表达无相关性(r=0.157,P=0.461)。结论直肠癌癌组织内P16和Ki-67水平表达与直肠癌临床病理特征密切相关,可能为直肠癌的临床治疗及预后评估提供参照。

Objective To investigate the relationship between the expression of multipletumor-suppressor gene1 P16 and proliferating cellnuclear antigen Ki-67 and the prognosis of rectal cancer patients.Methods From August 2016 to December 2018,postoperative tumors and adjacent normal tissues of 60 patients with rectal cancer were selected from the First People′s Hospital of Xiaoshan District of Hangzhou,Zhejiang Province.Immunohistochemical method was used to detect the expression of P16 and Ki-67.The positive rates of P16 and Ki-67 in different ages,pathological types,TNM stages,degree of differentiation and lymph node metastasis were observed.The survival of P16 and Ki-67 positive patients and negative patients one year after surgery was compared.Finally,the correlation between P16 and Ki-67 was analyzed by Spearman coefficient.Results The positive rates of P16 and Ki-67 in colorectal cancer tissues were significantly higher than that in paracancer normal tissues(P<0.05).TNM staging in patients with stageⅢ,high degree of differentiation P16 and Ki-67 were significantly higher than that of TNM stageⅡ,low-differentiation in patients(P<0.05);the Ki-67 positive rate of patients with lymph node metastasis was significantly higher than that of patients without lymph node metastasis,and the P16 positive rate of patients with lymph node metastasis was significantly higher than that of patients without lymph node metastasis(all P<0.05).The survival rate of P16 negative patients was significantly higher than that of positive patients(P<0.05).The one-year survival rate of Ki-67 positive patients was significantly lower than that of negative patients(P<0.05).There was no correlation between the expression of P16 and Ki-67 in rectal cancer tissues(r=0.157,P=0.461).Conclusion The expression levels of P16 and Ki-67 in rectal cancer tissues are closely related to the clinicopathological features of rectal cancer,which may provide reference for the clinical treatment and prognosis evaluation of rectal cancer.