UPLC-MS-MS快速测定烟草中131种农药残留量

Determination of 131 Pesticide Residues in Tobacco by UPLC-MS-MS

采用QuEChERS-超高效液相色谱-串联质谱法建立了烟草中131种农药残留的同时测定方法,样品用1%乙酸-乙腈萃取,氯化钠和无水硫酸镁盐析,经PSA固相分散吸附剂净化,采用正负离子多反应监测(MRM)模式检测,外标法定量。结果表明,131种农药在1~200μg/L范围内线性关系良好,决定系数(r^2)均大于0.99,方法的检出限为0.05~24.50μg/kg,131种目标物的平均回收率为65.3%~112.4%,RSD为3.2%~7.3%。该方法简便、快速、灵敏、可靠,适用于烟草中131种农药残留的快速测定。

An analytical method for simultaneous determination of 131 pesticide residues in tobacco was established by Qu EChERS-ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Tobacco samples were extracted with 1%(V/V)acetic acid-acetonitrile,salted out with sodium chloride and anhydrous magnesium sulfate,cleaned up with primary secondary amine PSA solide-phase dispersion,the extracts were then determined by UPLC-MS/MS in positive and negative ion modes and multiple reaction monitoring(MRM)mode,quantified by the external standard method.The results showed that all the 131 pescitides had good linearity in the ranges of 1-200μg/L,with the coefficients of determination(r2)higher than 0.99.The limits of detection(LOD)were from 0.05 to 24.50μg/kg.The average recoveries of all the pesticides were in the range of 65.3%-112.4%with the relative standard deviations between 3.2%to 7.3%.The method is simple,rapid,sensitive and reliable for determination of the 131 pesticide residues in tobacco.