电针对急性酒精性肝损伤小鼠肝脏表面血流灌注及氧化应激相关指标的影响

Effect of electroacupuncture on liver surface blood perfusion and related indexes of oxidative stress in mice with acute alcoholic liver injury

目的:观察电针对急性酒精性肝损伤小鼠肝脏表面血流灌注和氧化应激相关指标的影响,探讨电针治疗急性酒精性肝损伤的作用机制。方法:昆明小鼠按随机数字表法分为正常组、模型组、电针组,每组8只。以50%乙醇按6 g/kg灌胃1次,制备急性肝损伤模型。电针组给予"足三里""太冲"电针1次,持续30 min。用moorFLPI激光散斑成像仪动态观察各组小鼠肝表面血流灌注30 min的变化,用HE染色法观察肝组织形态学变化,用ELISA法检测小鼠肝组织超氧化物歧化酶(SOD)、丙二醛(MDA)含量。结果:与正常组比较,模型组小鼠肝索排列紊乱,肝窦变窄,肝细胞呈现肿胀、萎缩等形态学表现,肝脏表面血流灌注量、肝组织SOD含量显著降低(P<0.01,P<0.05),MDA含量显著升高(P<0.05)。与模型组比较,电针组小鼠肝索排列较为整齐,大致呈放射状排列,肝细胞肿胀、萎缩状况较轻,肝脏表面血流灌注量变化率明显增加(P<0.05),肝脏组织MDA含量显著降低(P<0.01)。结论:电针可以改善急性酒精性肝损伤小鼠肝脏表面血液循环,对该损伤所致氧化应激有一定调节作用。

Objective To observe the effect of electroacupuncture(EA)on liver surface blood perfusion and related indexes of oxidative stress in mice with acute alcoholic liver injury(AALI),so as to explore its mechanism underlying improving of AALI.Methods Twenty-four Kunming mice were randomly divided into control,model and EA groups(8 mice/group).AALI model was established by intragastric administration of 50%ethanol at 6 g/kg.EA(4-6 V,50 Hz)was applied to bilateral"Zusanli"(ST36)and"Taichong"(LR3)for 30 min.The liver surface blood perfusion was dynamically observed by moorFLPI laser speckle imager for 30 min.The morphological changes of the liver tissue were determined by H.E.staining.Levels of superoxide dismutase(SOD)and malondialdehyde(MDA)in the liver were measured by ELISA.Results Compared with the control group,the model group had markedly pathological changes of the hepatic cords,hepatic sinuses and hepatocytes shown by H.E.staining,a significant reduction in liver surface blood perfusion and the content of SOD in liver tissue(P<0.01,P<0.05),and a significant increase in the content of MDA in liver tissue(P<0.05).Compared with the model group,H.E.staining showed reduced pathological changes of the liver tissue,meanwhile the liver surface blood perfusion significant increased(P<0.05)and the content of MDA in liver tissue significantly decreased(P<0.01)in the EA group.Conclusion EA can improve the liver surface circulation of AALI mice,and regulate the oxidative stress response,and thus help to repair the damage of liver tissue.