电针对多囊卵巢综合征大鼠自噬相关因子的影响

Effect of electroacupuncture on levels of serum sex hormones and expression of ovarian autophagy related factors in rats with polycystic ovary syndrome

目的:观察电针对多囊卵巢综合征(PCOS)大鼠PI3K/AKT通路介导的自噬及性激素的影响,探讨电针治疗PCOS排卵障碍性不孕的可能机制。方法:SD大鼠随机分成对照组、模型组、电针组,每组10只。使用来曲唑灌胃21 d制备PCOS大鼠模型。电针组电针大鼠双侧"三阴交""太冲",每次20 min,每天1次,连续14 d。HE染色观察卵巢形态结构,ELISA法检测血清雄激素(T)、促黄体生成素(LH)、抗缪勒氏管激素(AMH)水平,Western blot法检测卵巢组织磷脂酰肌醇3-激酶(PI3K)、蛋白激酶B(AKT)、微管相关蛋白1轻链3(LC3)蛋白表达水平。结果:与对照组比较,模型组T、LH、AMH水平显著升高(P<0.05),PI3K和AKT表达明显降低(P<0.05),LC3Ⅱ/Ⅰ明显升高(P<0.05)。与模型组比较,电针组T、LH、AMH水平显著降低(P<0.05),PI3K和AKT表达水平明显升高(P<0.05),LC3Ⅱ/Ⅰ明显降低(P<0.05)。结论:电针可能通过上调PCOS大鼠卵巢组织中PI3K和AKT的蛋白表达进而降低卵巢组织自噬水平,从而改善其性激素水平及卵泡的生长发育状态,这可能是电针治疗PCOS排卵障碍性不孕的机制之一。

Objective To observe the effect of electroacupuncture(EA)on the expression of autophagy mediated PI3 K/AKT pathway and sex hormones in polycystic ovary syndrome(PCOS)rats,so as to explore the mechanisms underlying improvement of PCOS anovulatory infertility.Methods Six-week-old female Sprague-Dawley rats were randomly divided into control,model and EA groups(n=10 per group).The PCOS model was established by gavage of Letrozole solution(1.0 mg/kg),once daily for 21 consecutive days.Then,EA(2 Hz,1 mA)was applied to the"Sanyinjiao"(SP6)and"Taichong"(LR3)for 20 min,once a day for 14 successive days.The morphological changes of the ovary were observed after H.E.staining.The contents of serum androgen(T),luteinizing hormone(LH)and anti-mullerian hormone(AMH)were detected by ELISA.The expression levels of phosphatidylinositol 3-kinase(PI3 K),protein kinase B(AKT)and microtubule-associated protein 1 light chain 3(LC3)in the ovary tissues were detected by Western blot.Results Compared with the control group,the levels of serum T,LH and AMH contents,and ovarian LC3Ⅱexpression and the ratio of LC3Ⅱ/Ⅰwere significantly increased(P<0.05),and the expression levels of PI3 K and AKT proteins were considerably decreased in the model group(P<0.05).After EA intervention,the levels of serum T,LH,AMH,and the ratio of LC3Ⅱ/Ⅰwere considerably down-regulated(P<0.05),and those of PI3 K and AKT were obviously up-regulated in the EA group(P<0.05).Conclusion EA intervention can reduce serum T,LH and AMH contents,and the ratio of ovarian LC3Ⅱ/Ⅰand up-regulate ovarian PI3 K and AKT in PCOS rats,which may contribute to its effect in improving anovulatory infertility by suppressing autophagy.