小鼠过敏性哮喘模型肺组织中Th9细胞相关因子的变化及意义

Changes and significance of Th9 associated molecule in lung tissue of mouse model of allergic asthma

目的分析小鼠过敏性哮喘模型肺组织中各细胞因子表达水平的变化,探讨它们在过敏性支气管哮喘气道炎症中的调节机制。方法选取雌性BALB/c小鼠,复制小鼠过敏性哮喘模型,提取小鼠肺组织RNA,采用荧光定量PCR检测IL-9、IL-9R和PU.1 mRNA表达水平;收集支气管灌洗液,ELISA法检测灌洗液上清中各细胞因子(IL-4、INF-γ、IL-9和OVA特异IgE)的含量,并进一步分析这些分子间的相关关系。结果两组小鼠的Penh%随Mch剂量的增加都有升高的趋势,但是模型组小鼠上升的更快;与对照组相比,模型组小鼠BALF上清液中IL-4、IL-9、OVA特异抗体IgE水平显著升高(P<0.05),INF-γ水平则显著下降(P<0.05);模型组小鼠肺组织中IL-9、IL-9R及PU.1 mRNA相对表达水平与对照组相比均增高;除INF-γ与IL-9呈负相关关系,其他因子间均呈显著的正相关系。结论小鼠过敏性哮喘模型肺组织中Th9细胞相关因子(IL-9、IL-9R和PU.1)表达水平显著升高,可能在哮喘气道炎症进程中起到重要的免疫调节作用,是过敏性哮喘诊断与治疗的潜在靶点。

Objective Changes of various cytokines in lung tissue of mouse model of allergic asthma were analyzed and their regulatory mechanism in airway inflammation of allergic bronchial asthma was discussed.Methods Female BALB/c mice were selected to replicate the mouse model of allergic asthma,and detected the expression of IL-9,IL-9R and PU.1 in the lung tissue.The levels of IL-4,INF-γ,IL-9 and OVA specific IgE in BALF were measured.The correlation between these molecules was further analyzed.Results Penh%increased with the increase of Mch dose in both groups,but the model group increased faster.Compared with the control group,IL-4,IL-9 and OVA specific antibody IgE levels in BALF supernatant of mouse in the model group were significantly increased,while INF-γwere significantly decreased.The relative expression levels of IL-9,IL-9R and PU.1 mRNA in lung tissues of mouse in the model group were higher.Except INF-γand IL-9 have a negative correlation,other factors have a significant positive correlation.Conclusion The expression levels of IL-9,IL-9R and transcription factor PU.1 in lung tissue of mouse model of allergic asthma increased significantly,which may play an important regulatory role in the airway inflammation of asthmatic mice.