协同刺激分子OX40/OX40L在强直性脊柱炎患者外周血中的表达

Expression of OX40/OX40L in peripheral blood of patients with ankylosing spondylitis

目的探讨协同刺激分子OX40及其配体OX40L在强直性脊柱炎(AS)患者外周血单个核细胞(PBMC)中的表达及其临床意义。方法收集2017年10月~2019年10月我院收治的AS患者血液标本94例(AS组),其中稳定期患者52例,活动期患者42例,另取门诊同期健康体检者血液标本60例为对照组。分离PBMC,采用流式细胞仪检测OX40和OX40L在CD4^+T细胞和CD19^+ B细胞表面的表达变化,收集受试者所有临床检查资料,采用Spearman法分析PBMC中OX40和OX40L表达与AS患者Bath强直性脊柱炎指数评分(BASDAI)、Bath强直性脊柱功能指数(BASFI)、高敏C反应蛋白(hs-CRP)、血细胞沉降率(ESR)及球蛋白(GLB)水平的相关性。结果稳定期、活动期AS患者与对照组年龄、性别、BMI、吸烟史比例比较,差异无统计学意义(P>0.05)。与对照组比较,稳定期、活动期AS患者血清hs-CRP水平、外周血WBC、N、ESR、GLB水平、CD4^+T细胞表面OX40、CD19^+ B细胞表面OX40L表达率依次增加(均P<0.05)。外周血中CD4^+T/OX40表达率、CD19^+ B/OX40L表达与AS患者BASDAI、BASFI、血清hs-CRP、外周血N、ESR呈正相关(均P<0.05);外周血中CD4^+t/OX40、CD19^+ B/OX 40L表达与GLB无明显相关性(均P>0.05)。结论协同刺激分子OX40/OX40L在AS患者外周血中表达增加,可能与临床分期及疾病活动度有关。

Objective To study the expressions and clinical significance of costimulatory molecules OX40 and its ligand OX40L in peripheral blood mononuclear cells(PBMC)of patients with ankylosing spondylitis(AS).Methods Blood samples of 94 AS patients(AS group)were collected from October 2017 to October 2019,including 52 stable patients and 42 active patients.60 blood samples of healthy persons in the same period were taken as the control group.The PBMC was separated,and the expressions of OX40 and OX40L on CD4^+T cells and CD 19+B cells were detected by flow cytometry.The clinical examination data were collected,and the correlations between the expressions of OX40 and OX40L in PBMC and the levels of Bath Ankylosing Spondylitis disease activity index(BASDAI),Bath Ankylosing Spondylitis functional index(BASFI),high-sensitivity C-reactive protein(hs-CRP),erythrocyte sedimentation rate(ESR),globulin(GLB)in AS patients were analyzed by Spearman method.Results There was no significant difference in age,gender,BMI and smoking history between stable and active period AS groups and control group(P>0.05).Compared with the control group,the level of hs-CRP in serum,levels of WBC,N,ESR,GLB in peripheral blood,expression rates of OX40 on the surface of CD4^+T cells,OX40L on the surface of CD 19+B cells,and the scores of BASDAI and BASFI in the AS group increased successively(P<0.05).The expression rate of CD4^+T/OX40 in peripheral blood was positively correlated with BASDAI,BASFI,hs-CRP in serum,N and ESR in peripheral blood of AS patients(P<0.05).The expression of CD 19+B/OX40L was positively correlated with BASDAI,BASFI,hs-CRP in serum,N and ESR in peripheral blood of AS patients(P<0.05).The expression of CD4^+T/OX40,CD19^+ B/OX40L in peripheral blood was not significantly correlated with GLB(P>0.05).Conclusion The expression of costimulatory molecule OX40/OX40L is increased in peripheral blood of as patients,which may be related to clinical stage and disease activity.