中药饮片吸水率变化规律及中药复方汤剂相对密度预测模型研究

Change laws of water absorption in Chinese herbal pieces and prediction model of relative density for Chinese medicine decoction

该研究旨在探讨中药饮片吸水率变化规律,并建立中药复方汤剂相对密度预测模型。首先通过研究12组(按入药部位分组)共36种常用饮片吸水率随煎煮时间变化规律,建立了吸水率对煎煮时间的拟合方程,其中矿物类和其他类饮片的拟合方程r分别为0.6912和0.6633,其余10组饮片的拟合方程r为0.8022~0.9254,均P<0.05(n=21)。结合测定的饮片煎煮后吸水率,优化加水量公式,得液率可控制在100%±10%。其次,通过液体密度测定仪的方法学考察,并与比重瓶法的测定结果对比,确定液体密度测定仪可用于中药汤剂相对密度的快速检验。通过测定分析46种常用饮片煎煮后的得液量、相对密度及出膏率等相关参数,建立校正的相对密度(y)对出膏率(%,x)的线性回归方程y=0.0413x+1.0037,r=0.9309(P<0.01,n=46),线性范围1.94%~65.75%。基于此构建了中药复方汤剂相对密度预测模型雏形,经验证,8个复方汤剂的相对密度在此模型的预测区间范围内。该研究为构建中药煎药数据库,实现个性化煎药和中药汤剂快速质控奠定基础。

This study aims to explore the change laws of water absorption in Chinese herbal pieces and establish the prediction model of relative density for Chinese medicine compound decoction.Firstly,fitted equations of water absorption and decocting time was established by observing the change laws of water absorption in 36 kinds of Chinese herbal pieces in 12 groups(according to the drug-parts)with decocting time.The r value of the mineral group and other type group was 0.6912 and 0.6633,respectively.The r value of the remaining 10 groups was 0.8022-0.9254.All P values were less than 0.05(n=21).The formula of the amount of water added was optimized by combining the fitted equations with determined water absorption,and the liquid yield could be controlled in a range of 100%±10%.Secondly,it was determined that the liquid density tester could be used for the rapid determination of relative density of Chinese medicine decoction after methodological study and comparison with the pycnometer method.The linear regression equation between the corrected relative density(y)and extraction ratio(%,x)was built by measuring and analyzing the related parameters such as liquid yield,relative density and extraction ratio in 46 kinds of Chinese herbal pieces.The established equation was y=0.0413x+1.0037,r=0.9309(P<0.01,n=46),with linear range of 1.94%-65.75%.Based on this,the prototype model for predicting relative density of Chinese medicine decoction was established,and the relative densities of 8 Chinese medicine decoctions were within the prediction interval of this model in verification.This study lays a foundation for database construction of Chinese medicine decoction,implementation of personalized decocting mode and rapid quality control of Chinese medicine decoction.