对一起汉滩病毒感染案例44年后长时效体液免疫应答的回顾性分析

Retrospective analysis of long-term immunity protection in a case of hantaan virus infection after 44 years

目的探讨汉滩病毒(hantaan virus,HTNV)感染者长时效体液免疫应答情况,为研究长时效体液免疫应答规律提供参考依据。方法采集4名HTNV感染者感染44年后的血清,通过酶联免疫吸附试验和HTNV中和抗体检测技术检测分析患者血清中HTNV特异性抗体IgM、IgG及中和活性抗体滴度。结果4名HTNV感染者血清中均检测到不同滴度的HTNV特异性IgG抗体,其滴度最高可达1∶800,HTNV特异性IgM抗体均未检测到。此外,在血清中也检测到不同滴度的中和活性抗体,其滴度最高可达1∶10。结论HTNV感染44年后体内仍存在长时效体液免疫应答,其可以为感染者提供终身免疫保护。

Objective To investigate the long-term humoral immune response in patients infected with hantaan virus(HTNV)and provide references for the study of long-term humoral immune response.Methods The sera from 4 patients infected with HTNV were collected after 44 years.The titers of HTNV-specific antibodies IgM and IgG as well as the neutralization antibody in their sera were analyzed by using enzyme linked immunosorbent assay and neutralization antibody test.Results Among the 4 HTNV-infected patients,the presence of different titers of HTNV-specific IgG antibodies were detected in the sera,and the titer was up to 1∶800.But the HTNV-specific IgM antibodies were not detected.In addition,different titers of neutralization antibodies were also detected in their sera,the titer was up to 1∶10.Conclusions There is a long-lasting humoral immune response in the body after 44 years of HTNV infection,and it provides a lifelong immune protection for the infected patients.