滤泡调节性T细胞与HIV感染疾病进展关系的研究

Relationship between follicular regulatory T cells and HIV infection progression

目的探索滤泡调节性T细胞(follicular regulatory T cell,TFR)与HIV感染疾病进展的关系。方法以2015—2019年于首都医科大学附属北京佑安医院门诊就诊及随访的47例HIV感染者(包括未治疗HIV感染者,抗病毒治疗免疫重建成功和免疫重建失败患者)及9例健康体检者作为研究对象。将CD3^+CD4^+CXCR5^+Foxp3^+的细胞定义为TFR,分析HIV感染者TFR数量与CD4^+T细胞计数及病毒载量的关系,并对比分析不同疾病阶段患者TFR数量的差异。结果TFR数量与CD4^+T细胞计数呈正相关(r=0.567,P=0.002),与HIV载量呈负相关(r=-0.394,P=0.042);HIV感染者的TFR数量明显低于健康体检者,免疫重建成功的患者TFR数量明显高于免疫重建失败的患者。结论随着HIV感染疾病的进展,TFR数量逐渐降低。

Objective To explore the relationship between follicular regulatory T cells(TFR)and the progression of HIV infection.Methods A total of 47 HIV-infected patients(including untreated HIV-infected patients,patients with successful immune reconstruction,patients with unsuccessful immune reconstruction after antiretroviral therapy)who were treated and followed up at Beijing YouAn Hospital,Capital Medical University from 2015 to 2019 and 9 healthy physical examinees were enrolled in this study.The CD3^+CD4^+CXCR5^+Foxp3^+cells were defined as TFR.The relationship of the absolute number of TFR with the CD4^+T cell count and viral load of HIV-infected patients was analyzed,and the differences in the number of TFR at different disease stages were compared and analyzed.Results The TFR count was positively correlated with CD4^+T cell count(r=0.567,P=0.002),and negatively correlated with HIV viral load(r=-0.394,P=0.042);TFR levels of HIV-infected patients were significantly lower than that of healthy physical examinees.Patients with successful immune reconstruction had a significantly higher TFR level than patients with unsuccessful immune reconstruction.Conclusions As HIV infection progresses,TFR level gradually decreases.