多波长HPLC同时测定丹七粉中7个有效成分的含量

Simultaneous Determination of Seven Effective Ingredients of Danqi Powder by Multi-Wavelength HPLC

目的:建立多波长HPLC同时测定丹七粉中7个有效成分的方法。方法:色谱柱为Waters SunFire C18柱(4.6 mm×250 mm,5μm),以乙腈(A)-0.05%磷酸水溶液(B)为流动相进行梯度洗脱:0~18 min,A相比例为10%~18%;18~27 min,A相比例为18%~25%;27~50 min,A相比例为25%~80%;50~65 min,A相比例为80%~80%;检测波长为203 nm、270 nm和286 nm,柱温为30℃。结果:三七皂苷R1、人参皂苷Rg1、丹酚酸B、人参皂苷Rb1、隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA分别在0.21~8.20μg、1.01~40.40μg、1.02~40.80μg、0.51~20.40μg、0.06~2.40μg、0.03~1.20μg、0.08~3.20μg范围内,其进样量与峰面积的线性拟合度和加样回收率均良好。结论:该方法可同时测定丹七粉的主要有效成分,结果稳定,重复性好。

Objective:To establish a multi-wavelength HPLC method for the simultaneous determination of seven effective components of Danqi powder.Method:The chromatographic column is a Waters SunFire C18 column(4.6 mm×250 mm,5 μm),with acetonitrile(A)-0.05% phosphoric acid aqueous solution(B)as the mobile phase for gradient elution:0~18 min,10%~18%A;18~27 min,18%~25% A;27~50 min,25%~80%A;50~65 min,80%~80%A;detection wavelengths were 203 nm,270 nm and 286 nm,column temperature was 30 ℃.Results:Panax notoginsenoside R1,ginsenoside Rg1,salvianolic acid B,ginsenoside Rb1,cryptotanshinone,tanshinone Ⅰ and tanshinone ⅡAthe range respectively were 0.21~8.20 μg,1.01~40.40 μg,1.02~40.80 μg,0.51~20.40 μg,0.06~2.40 μg,0.03~1.20 μg,0.08~3.20 μg,it showed good linear relationship and recovery rate from example size and peak area.Conclusion:This method can simultaneously determine the content of the main active ingredients of Danqi powder,with stable results and good repeatability.