四君子颗粒含药血清对棕榈酸诱导的MIN6细胞凋亡的影响

Effects of Sijunzi Granules medicated serum on MIN6 cell apoptosis induced by palmitic acid

目的探讨四君子颗粒含药血清对棕榈酸诱导的MIN6细胞凋亡的影响。方法SD大鼠灌胃给予四君子颗粒(10.5、5.25 g/kg)7 d,制备四君子颗粒含药血清。四君子颗粒含药血清预处理MIN6细胞24 h后,加入0.4 mmol/L棕榈酸孵育MIN6细胞24 h。CCK-8法检测细胞活力,流式细胞术检测细胞凋亡,Western blot法检测GRP78、ATF4、CHOP、p-PERK蛋白表达。结果四君子颗粒含药血清减轻了棕榈酸诱导的MIN6细胞损伤和凋亡(P<0.05,P<0.01),下调内质网应激相关蛋白GRP78、ATF4、CHOP蛋白表达,降低PERK蛋白磷酸化(P<0.05,P<0.01)。结论四君子颗粒含药血清可有效减轻棕榈酸诱导的MIN6细胞损伤和凋亡,其机制可能与抑制内质网应激相关的信号通路有关。

AIM To investigate the effects of Sijunzi Granules(SGs)medicated serum on MIN6 cell apoptosis induced by palmitic acid.METHODS SD rats were given 7-day intragastric SGs administration(10.5,5.25 g/kg)for preparation of SGs medicated serum.MIN6 cells pretreated with 24 h SGs serum were then exposed to 0.4 mmol/L palmitic acid induction for 24 h.The MIN6 cells were subsequently subjected to the detection of cell viability by CCK-8 method,investigation of cell apoptosis by flow cytometry,and detection of GRP78,ATF4,CHOP,and p-PERK protein expression by Western blot.RESULTS SGs medicated serum alleviated injury and apoptosis of palmitic acid-induced MIN6 cells(P<0.05,P<0.01),and down-regulated the expression of endoplasmic reticulum stress-related proteins GRP78,ATF4,and CHOP expression,and reduced PERK protein phosphorylation as well(P<0.05,P<0.01).CONCLUSION SGs medicated serum can effectively reduce MIN6 cells injury and apoptosis induced by palmitic acid-induced,and its mechanism may be related to the inhibition of endoplasmic reticulum stress-related signaling pathways.