不同产地黄芪中山奈素的测定方法研究

Study on the Determination Method of Kaempferol in Astragalus membranaceus from Different Producing Areas

目的:测定不同产地黄芪中山奈素的含量。方法:采用HPLC色谱法,色谱条件为:Thermo SCIENTIFIC Hypersil GOLD Dim.(mm)(4.6mm×250mm,5μm),以流动相:甲醇-0.1%磷酸(58∶42)进行洗脱,流速为1.0mL/min,柱温为30℃,检测波长为370nm,进样量为10μL。结果:不同产地的黄芪中山奈素含量存在一定差异,内蒙古产的黄芪中山奈素含量较高,而河北产的黄芪中山奈素含量最低。结论:通过对不同产地的黄芪进行含量测定研究,确定了质量分析研究方法,该方法专属性强,重现性好,方法简单,可为黄芪药材的质量控制提供依据。

Objective:The content of kaempferol in Astragalus membranaceus from different origins was determined.Methods:Using HPLC chromatography,the chromatographic conditions are Thermo SCIENTIFIC Hypersil GOLD Dim.(mm)(4.6 mm×250 mm,5μm),and the mobile phase is methanol-0.1% phosphoric acid(58∶42)for elution,and the flow rate is 1.0 mL/min,column temperature is 30℃,detection wavelength is 370 nm,injection volume is 10 uL.Results:There are certain differences in the content of kaempferol in Astragalus membranaceus from different producing areas.Astragalus membranaceus from Nei Mongol has a higher content of kaempferol,while Astragalus membranaceus from Hebei Province has the lowest content.Conclusion:Through the determination of the content of Astragalus membranaceus from different origins,the quality analysis research method was determined.This method has strong specificity,good reproducibility and simple method,which can provide a basis for the quality control of Astragalus membranaceus medicinal materials.