Detection of tuberculosis genes associated with drug-resistance in paraffin-embedded tissue specimens using next generation sequencing technology

Objective To evaluate the use of multiplex PCR amplicon sequencing(mPCR-NGS)technology in detecting gene mutations related to drug resistance of Mycobacterium tuberculosis(MTB)in formalin-fixed paraffin-embedded tissue specimens,and to explore its clinical value in the diagnosis of drug-resistant tuberculosis.Methods Fifty clinical MTB strains isolated in the Changping District Tuberculosis Control Institute of Beijing from April 2013 to October 2015 with drug susceptibility test(DST)results of rifampicin,isoniazid,ethambutol,streptomycin,ofloxacin,capreomycin,kanamycin and amikacin available were recovered,including 42 drug-resistant strains and 8 drug-sensitive strains.