钟状期小鼠上颌第一磨牙的拔除与原位移植模型

Extraction and orthotopic transplantation model of the upper first molar at bell stage in mice

目的:为研究牙再生、牙根发育和牙萌出建立能模拟天然牙发育环境的动物模型。方法:选取钟状期小鼠上颌第一磨牙作为牙拔除与牙移植的研究对象,进行动物建模,并通过术后影像学和组织学观察与肾被膜下移植模型进行对比。结果:出生后第8天的小鼠上颌第一磨牙牙胚处于牙冠基本形成而牙根即将开始发育的阶段,适合用于本模型。相较肾被膜下移植模型,本模型移植牙胚的牙根发育更接近天然牙发育。结论:钟状期小鼠上颌第一磨牙牙胚拔除及移植模型是观察牙根发育和牙萌出的良好选择。

Objective:Establishing an animal model which can simulate the natural tooth developing environment in physiological condition for the researches of tooth regeneration,root development and tooth eruption.Methods:The upper first molar of mouse at bell stage was used in this model for extraction and transplantation,comparing with the subrenal capsule transplantation model by radiological and histological observation postoperatively.Results:The tooth germ of upper first molar by the postnatal 8th day has almost formed the crown and the root development was about to begin,which was a suitable subject of study in this model.Comparing with that in the subrenal capsule transplantation model,the root development of transplanted tooth germ in this model showed more similar to that of the natural tooth.Conclusions:The extraction and orthotopic transplantation model of the upper first molar at bell stage in mice is a desired option for the observation of root development and tooth eruption.