诺氟沙星磁分子印迹纳米粒子的制备及其富集分离

Preparation of Magnetic Molecular Imprinted Nanoparticles for Enrichment and Separation to Norfloxacin

以氨基修饰的Fe3O4纳米粒子(Fe3O4@NH2NPs)为载体、诺氟沙星(norfloxacin,NOR)为模板分子、多巴胺(dopamine,DA)为功能单体和交联剂,通过优化制备条件获得特异性识别NOR的磁性分子印迹纳米粒子(magnetic molecularly imprinted polymers nanoparticles,MMIPs NPs)。结果表明,当溶剂乙醇-水体积比为1∶1,Fe3O4@NH2NPs、NOR、DA添加量分别为500、15、134 mg,洗脱液乙醇-乙酸溶液体积比为97∶3时,制备的MMIPs NPs对NOR的吸附效率最高。当4 mg MMIPs NPs吸附1 mL 0.02 mg/mL的NOR 60 min时,其吸附效率为94.61%,相对标准偏差为0.79%,是磁性非分子印迹纳米粒子(magnetic non-molecular imprinted polymers nanoparticles,MNIPs NPs)的2.18倍。当15 mg MMIPs NPs吸附1 mL 0.1 mg/mL NOR 60 min时,其吸附效率可达到99.17%,相对标准偏差为1.03%。

Magnetic molecularly imprinted polymers nanoparticles(MMIPs NPs) with specific identification toward norfloxacin(NOR) were prepared using Fe3 O4@NH2 NPs as carrier,NOR as template molecule,dopamine(DA)as functional monomer and crosslinking agent. The synthesis condition of MMIPs NPs were optimized,and the adsorption ability of MMIPs NPs toward NOR were studied,which provided a technique for the rapid and efficient enrichment and separation of NOR residues in the food sample. The results showed that the obtained MMIPs NPs displayed the highest adsorption ability for NOR when the volume ratio of ethanol-water(solvent)was 1 ∶ 1,the addition of Fe3 O4@NH2 NPs,NOR and DA were 500 mg,15 mg and 134 mg respectively,and the volume ratio of the ethanol-acetic acid(elution solution)was 97 ∶ 3. The adsorption efficiency reached 94.61 % for 1 mL NOR of 0.02 mg/mL when 4 mg MMIPs NPs were used and adsorption time was 60 min. The adsorption capacity of MMIPs NPs toward NOR was 2.18 times than that of magnetic non-molecular imprinted polymers nanoparticles(MNIPs NPs). The adsorption efficiency of MMIPs NPs reached99.17 % for 1 mL NOR of 0.1 mg/mL when 15 mg MMIPs NPs were used and adsorption time was 60 min with relative standard deviation of 1.03 %.