摘要
目的:研究去甲斑蝥素对人雄激素非依赖型前列腺癌PC-3细胞生长抑制与诱导凋亡的作用。方法:CCK-8技术检测去甲斑蝥素对PC-3细胞生长抑制率的影响,流式细胞仪检测去甲斑蝥素对PC-3细胞周期和凋亡率的影响。结果:去甲斑蝥素对PC-3细胞有生长抑制作用,随去甲斑蝥素浓度升高,抑制作用增强,呈现剂量-效应关系。流式细胞检测提示,去甲斑蝥素作用于PC-3细胞后,G 0/G 1期细胞明显减少,S、G 2期PC-3细胞增加,凋亡率升高(P<0.05)。结论:去甲斑蝥素能抑制人雄激素非依赖型前列腺癌PC-3细胞的增殖,促进PC-3细胞凋亡。
Objective:To investigate the effect of norcantharidin on proliferation and apoptosis of human androgen independent prostate cancer cell line PC-3.Methods:CCK-8 method was used to detect the growth inhibition rate of PC-3 cells,and flow cytometry was used to detect the cell cycle and the apoptosis rate of PC-3 cells.Results:Norcantharidin could inhibit the growth of PC-3 cells.With the increase of norcantharidin concentration,the effect increased in a dose-response relationship.Flow cytometry showed that after norcantharidin treated PC-3 cells,G 0/G 1 phase cells decreased,S and G 2 phase cells increased,apoptosis rate increased(P<0.05).Conclusion:Norcantharidin can significantly inhibit the proliferation of human androgen independent prostate cancer cell line PC-3 and induce it apoptosis.
作者
雒向宁
王文娟
李凯
杜宏宏
LUO Xiangning;WANG Wenjuan;LI Kai(epartment of Urology,Affiliated Hospital of Shaanxi University of Chinese Medicine,Xianyang 712000)
出处
《陕西医学杂志》
CAS
2020年第8期928-931,939,共5页
Shaanxi Medical Journal
基金
全国西学中骨干人才培训项目(2019269)
陕西省中医药管理局资助项目(JCMS026)。
关键词
去甲斑蝥素
前列腺癌
凋亡
细胞培养
增殖
流式细胞术
Norcantharidin
Prostate cancer
Apoptosis
Cell culture
Proliferation
Flow cytometry
