一测多评法测定瘪桃干药材中3个酚酸类成分

Simultaneous determination of three phenol acids in Amygdali Persicae Fructus by QAMS

目的:建立同时测定瘪桃干中绿原酸、新绿原酸、隐绿原酸的HPLC方法,在此基础上建立一测多评法,验证该方法在瘪桃干质量控制中应用的可行性和技术适用性。方法:采用C 18色谱柱,流动相为乙腈-0.1%磷酸水溶液(8∶92);流速:1.0 mL·min^-1;柱温:35℃;测定波长:327 nm,以瘪桃干中绿原酸为指标,建立其与新绿原酸、隐绿原酸间的相对校正因子,并用该相对校正因子计算隐绿原酸、新绿原酸的量,对一测多评法与外标法进行比较。结果:建立了瘪桃干中3个酚酸类成分一测多评法方法学的考察模式;一测多评法对不同的仪器、不同色谱柱的结果没有显著性差异。结论:同时测定绿原酸、新绿原酸、隐绿原酸3个酚酸类成分的一测多评法方法可靠,结果准确,可用于控制瘪桃干的质量。

Objective:To establish an HPLC method for simultaneous determination of chlorogenic acid,neochlorogenic acid and cryptochlorogenic acid in Amygdali Persicae Fructus and to validate the feasibility and technical suitability of quantitative analysis of multi-components by single marker(QAMS)for quality control of Amygdali Persicae Fructus.Methods:The test was performed on C 18 column under isocratic elution of acetonitrile-0.1%phosphoric acid solution(8∶92).The flow rate was 1.0 mL·min^-1,the detection wavelength was 327 nm,and the column temperature was 35℃.Chlorogenic acid,the main component in Amygdali Persicae Fructus,was used as an indicator,a relative correction factors between chlorogenic acid and other phenol acids were established.Then the correction factor was used to calculate the contents of the other two phenol acids in Amygdali Persicae Fructus.The calculated results were compared with that of the external standard method.Results:The QAMS method of three phenol acids in Amygdali Persicae Fructus was established.There was no significant difference compared with the external standard method(ESM)when the different instruments and columns were used.Conclusion:The established QAMS method for simultaneous determination of chlorogenic acid,neochlorogenic acid and cryptochlorogenic acid in Amygdali Persicae Fructus is reliable and accurate.It can be used for quality control of Amygdali Persicae Fructus.