摘要
Dendritic cell(DC)vaccine is an effective strategy for cancer immunotherapy by carrying antigen into DCs and migrating these DCs to drain lymph nodes after inoculation.In this article,second near-infrared window(NIR-II)fluorescent nanoparticles have been used to uptake antigen and activate DCs.Ovalbumin(OVA),an antigen for immunization,can be loaded on the surface of these NIR-II fluorescent nanoparticles via electrostatic interaction by virtue of their functionalized poly(L-lysine)(PLL),which exhibits biocompatibility and strong selective interaction with OVA.In addition,these antigen-loaded complexes can efficiently be engulfed by immature DCs to induce DC maturation and cytokine secretion.After subcutaneous injection,highly sensitive NIR-II fluorescence signal from nanoparticles indicates that nanoparticle-labeled DCs can successfully migrate into lymph nodes in vivo,showing great promise in immunotherapy against cancer.
基金
the National Natural Science Foundation of China(21574064,21674048,21671109,21604042)
Primary Research&Development Plan of Jiangsu Province(BE2016770)
Synergetic Innovation Center for Organic Electronics and Information Displays
the Priority Academic Program Development of Jiangsu Higher Education Institutions(YX030003)。
