不同龈下楔状缺损充填材料对牙龈卟啉单胞菌生物膜形成的影响

The effect of commonly used subgingival wedge-shaped defect filling materials on the formation of Porphyromonas gingivalis biofilm

目的:探讨常用龈下楔状缺损充填材料对牙龈卟啉单胞菌(P.gingivalis)生物膜形成的影响。方法:在48颗离体前磨牙颊侧牙颈部制备同一规格V类洞,将其随机分为A、B、C 3组,每组16颗,分别采用通用纳米固体树脂、通用纳米流体树脂、玻璃离子充填、抛光后,使用Isomet 4000精密切割机制成包含全部充填材料的试件(近远中径5 mm、龈径3 mm、颊舌向深度2 mm)。将上述3组试件置于P.gingivalis菌液中培养24 h,采用结晶紫染色法观察P.gingivalis的黏附量,使用激光共聚焦显微镜(confocal laser scanning microscopy,CLSM)观察P.gingivalis生物膜的形成情况。采用SPSS 22.0软件包对所得数据进行Kruskal-Wallis秩和检验。结果:结晶紫染色实验显示,玻璃离子表面黏附P.gingivalis的量显著少于通用纳米固体树脂与通用纳米流体树脂(P<0.05)。CLSM扫描可见,3组间P.gingivalis生物膜内的活菌比例及生物膜厚度无显著差异(P>0.05);玻璃离子表面P.gingivalis生物膜稀疏,呈小团块状,未聚集成片;通用纳米固体树脂组的P.gingivalis生物膜则呈大块片状,立体结构明显。结论:与通用纳米树脂材料相比,玻璃离子表面更不利于P.gingivalis生物膜的形成。对牙周健康而言,玻璃离子更适用于龈下楔状缺损的充填治疗。

PURPOSE:To investigate the effect of commonly used subgingival wedge-shaped defect filling materials on the formation of Porphyromonas gingivalis(P.gingivalis)biofilm.METHODS:Subgingival class V cavities were prepared on the buccal surfaces of 48 extracted premolars.After that,the premolars were randomly divided into 3 groups(A,B and C)with 16 in each group.After filling and polishing with universal nano fluid resin,universal nano solid resin and glass ionomer cement individually,Isomet 4000 precision cutting machine was used to make specimens containing all the filling materials(mesiodistal distance:5 mm,occlusal-gingival distance:3 mm,buccolingual distance:2 mm).All the specimens were placed in P.gingivalis bacterial solution for 24 hours to form P.gingivalis biofilm.Then the attachment amount of P.gingivalis was observed by crystal violet staining.Confocal laser scanning microscopy(CLSM)was used to observe the P.gingivalis biofilm.SPSS 22.0 software package was used for Kruskal-Wallis rank sum test.RESULTS:The results of crystal violet staining showed that the amount of P.gingivalis adhered to the glass ionomer cement filling surface was significantly less than that of other groups(P<0.05).CLSM scan showed no significant difference in proportion of viable bacteria and biofilm thickness among three groups(P>0.05).P.gingivalis biofilm in glass ionomer cement group was sparse,showing small clumps,and did not aggregate into pieces.On the surface of universal nano solid resin,P.gingivalis biofilm was in large pieces with a three-dimensional structure.CONCLUSIONS:Compared with the universal nano solid/fluid resin,the surface of glass ionomer cement is not conducive to the formation of P.gingivalis biofilm.Therefore,from the perspective of periodontal health,glass ionomer cement is suitable for filling treatment of subgingival wedge-shaped defects.