拟南芥ATGs在发育和非生物胁迫中的表达特征和功能分析

Prediction of the Function of Autophagy-Related Genes(ATGs) in Development and Abiotic Stress Based on Expression Profiling in Arabidopsis

植物细胞自噬(Autophagy)是依赖于液泡来降解体内异常蛋白的重要途径,其中ATGs (Autophagyrelated genes)蛋白对该途径中的核心组分自噬体的形成至关重要。目前在模式植物拟南芥中已经鉴定到35个ATGs,它们对细胞自噬的发生和调节起到关键作用,然而全面地认知植物ATGs在生长发育和逆境中的转录表达变化尚有不足。本研究通过收集公共数据库中的转录组数据,利用生物信息学方法挖掘了拟南芥ATGs的表达信息,展示了其在47个不同发育时期或组织部位的表达聚类情况,对比分析了ATGs在植物非生物胁迫情况下的地上部分(茎)和地下部分(根)的表达差异,同时结合ATGs的启动子组分分析,阐明ATGs表达的时空变化可能的调控分子机制。本研究将为进一步探索ATGs在生长发育和非生物胁迫中的功能提供基础数据和相关依据。

Autophagy is an important pathway that relies on vacuoles to degrade abnormal proteins in plant cells. ATGs(Autophagy-related genes) play a key role in the formation of autophagosomes in the core components of this pathway. 35 ATGs have been identified in Arabidopsis, which involve in the occurrence and regulation of autophagy. However, a comprehensive understanding of the gene expression of ATGs in plant growth and development, abiotic stress and its regulatory elements are still insufficient. This study uses bioinformatics methods to collect and process transcriptome data from public databases, we analyzed the expression profiles and cluster patterns of ATGs gene in 47 different tissues and organs under vegetative, reproductive and abiotic stresses in Arabidopsis, then predictive analysis of their possible functions in growth and stress environments,and further combined with promoter analysis of ATGs to explain the possible regulatory molecular mechanisms of ATGs gene expression. This study will provide supporting data and scientific basis for studying the molecular mechanism of ATGs’ function and regulation in the process of growth and abiotic stress in autophagy comprehensively.