高尔基磷蛋白3参与调控乳腺癌的增殖

Golgi Phosphoprotein 3 Is Involved in the Regulation of Breast Cancer Rroliferation

高尔基体磷蛋白3 (GOLPH3)是一种新的致癌基因,与某些恶性肿瘤的生长和转移密切相关。本研究旨在考察GOLPH3在乳腺癌细胞增殖中的作用。采用MTS和BrdU 2种方法检测GOLPH3对人乳腺癌细胞系MDA-MB-435S增殖的影响,流式细胞仪检测细胞周期,qRT-PCR检测细胞周期相关基因(Cyclin D, Cyclin E和P21)表达。研究发现,GOLPH3在乳腺癌样本中显著上调;GOLPH3的过表达显著促进MDA-MB-435S细胞的增殖;miRNA-590-3p的过表达抑制了细胞的GOLPH3 mRNA表达及增殖;过表达ATF-3通过抑制miR-590-3p来促进MDA-MB-435S细胞增殖。本研究表明抑制ATF-3/miR-590-3p/GOLPH3信号通路可抑制乳腺癌细胞的增殖,GOLPH3可能成为未来临床治疗中乳腺癌早期诊断的潜在生物标志物。

Golgi phosphoprotein 3(GOLPH3) is a new oncogene and is closely related to the growth and metastasis of some malignant tumors. This study was designed to investigate the role of GOLPH3 in breast cancer cell proliferation. The effects of GOLPH3 on the proliferation of human breast cancer cell line MDA-MB-435S were detected by MTS and Brd U assay. Cell cycle was detected by flow cytometry, and cell cycle related genes(Cyclin D,Cyclin E and P21) expressions were detected by q RT-PCR. The study found that GOLPH3 was significantly upregulated in breast cancer samples. Overexpression of GOLPH3 significantly promoted the proliferation of MDA-MB-435S cells. Overexpression of mi RNA-590-3p inhibited GOLPH3 mRNA expression and proliferation in cells. Overexpression of ATF-3 promoted MDA-MB-435S cell proliferation by inhibiting miR-590-3p. This study demonstrates that inhibition of ATF-3/miR-590-3p/GOLPH3 signaling pathway can inhibit breast cancer cell proliferation;GOLPH3 may be a potential biomarker for early diagnosis of breast cancer in future clinical treatment.