低强度激光治疗对游泳大鼠氧化应激和功能恢复的影响

Effects of Low-level Laser Therapy on Oxidative Stress and Functional Recovery in Swimming Rats

本研究的目的是确定低强度激光疗法(LLLT)联合有氧运动对机体氧化应激和心肺功能恢复的影响。本研究测试了60只SD大鼠,将大鼠随机分为对照组、低强度激光治疗组、运动组和低强度激光治疗+运动组(n=15)。检测了各组大鼠有氧运动训练或低强度激光治疗6周后的最大摄氧量(VO2max 0.75)和最大运动速度;检测了各组大鼠腓肠肌中过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)和丙二醛(MDA)水平以及大鼠血清肌酸激酶(CK)、天冬氨酸转氨酶(AST)和丙氨酸转氨酶(ALT)水平。研究显示,低强度激光治疗+运动组大鼠的最大摄氧量和最大运动速度均显著高于其他组(p<0.05)。低强度激光治疗+运动组的CAT、SOD和GPx活性均显著高于其他组(p<0.05),而MDA水平显著低于其他组(p<0.05)。低强度激光治疗+运动组的血清CK、AST和ALT水平均显著低于其他组(p<0.05)。本研究表明在有氧运动基础上进行低强度激光治疗可有效提高机体的心肺功能,改善抗氧化防御系统,减少脂质过氧化损伤和运动损伤。

The purpose of this study was to determine the effects of low-level laser therapy(LLLT) combined with aerobic exercise on oxidative stress and cardiopulmonary function recovery. In this study, 60 SD rats were randomly divided into control group, low-level laser treatment group, exercise group and low-level laser treatment +exercise group(n=15). The maximal oxygen uptake(VO2 max 0.75) and maximum exercise rate in rats after 6 weeks of aerobic exercise training or low-level laser treatment were measured. The levels of catalase(CAT), superoxide dismutase(SOD), glutathione peroxidase(GPx) and malondialdehyde(MDA) in the gastrocnemius muscle of each group were examined. Serum creatine kinase(CK), aspartate aminotransferase(AST) and alanine aminotransferase(ALT) levels were measured. Studies showed that the maximum oxygen uptake and maximum exercise rate in the low-level laser treatment+exercise group were significantly higher than the other groups(p<0.05). The activities of CAT, SOD and GPx in the low-level laser therapy+exercise group were significantly higher than those in the other groups(p<0.05), while the MDA level was significantly lower than the other groups(p<0.05). Serum CK, AST, and ALT levels in the low-level laser therapy+exercise group were significantly lower than the other groups(p<0.05).This study demonstrates that low-level laser treatment based on aerobic exercise can effectively improve the cardiopulmonary function of the body, improve the antioxidant defense system, and reduce lipid peroxidation damage and sports injuries.