UC患者IL-10基因rs1800871、rs1800872、rs1800896位点基因多态性分析

Genetic polymorphism analysis of IL-10 gene rs1800871,rs1800872 and rs1800896 site in UCpatients

目的分析溃疡性结肠炎(UC)患者白细胞介素-10(IL-10)基因rs1800871、rs1800872、rs1800896位点基因多态性。方法选择2014年1月—2018年12月本院收治的80例UC患者作为研究对象,均纳入UC组。另按1∶1比例筛选同期于本院行健康体检的非UC健康人群80名纳入对照组。聚合酶链式反应-限制性片段长度多态性分析方法(PCR-RFLP)检测其IL-10基因多态性,比较两组IL-10基因-819T/C(rs1800871)位点、-592A/C(rs1800872)位点、-1082G/A(rs1800896)位点的基因多态性分布情况。结果两组IL-10基因-819T/C(rs1800871)位点CC、CT、TT型基因分布、-592A/C(rs1800872)位点AA、AG、CC基因型分布情况比较,差异均无统计学意义(P>0.05);但-1082G/A(rs1800896)位点GG分布比重显著高于对照组,AA型基因比重显著低于对照组,差异有统计学意义(P<0.05)。结论 IL-10基因-1082G/A(rs1800896)位点或与UC密切相关,GG基因型或为UC的易感基因,AA型基因或为保护型基因,值得临床重视。

Objective To analyze the gene polymorphism of interleukin-10(IL-10) gene rs1800871, rs1800872 and rs1800896 site in patients with ulcerative colitis(UC).Methods The subjects of this study were 80 cases of UC patients admitted to our hospital from January 2014 to December 2018, all of the patients were included in the UC group. Another eighty cases of healthy people without UC were screened from those went for healthy check-upduring the same periodaccording to 1:1 proportion, and they were crowd into the control group. Polymerase chain reaction-restriction fragment length polymorphism analysis method(PCR-RFLP) was used to detected the IL-10 gene polymorphism.The polymorphic distribution of IL-10 gene at-819 T/C(rs1800871),-592 A/C(rs1800872) and-1082 G/A(rs1800896) was compared between the two groups.Results The distribution of CC, CT and TT genotypes at-819 t/C(rs1800871) and AA, AG and CC genotypes at-592 a/C(rs1800872) of IL-10 gene in the two groups showed no significant difference(P>0.05). However, the proportion of GG distribution at-1082 g/A(rs1800896) site was significantly higher than that in the control group, and the proportion of AA type gene was significantly lower than that in the control group, with statistically significant differences(P<0.05).Conclusions The IL-10 gene-1082 G/A(rs1800896) site is closely related to UC, the GG genotype is the susceptible gene of UC, the AA genotype is the protective gene, which is worthy of clinical attention.