中医药调控缺血缺氧微环境对骨髓间充质干细胞增殖、分化及衰老的影响

Effects of Chinese medicine on proliferation,differentiation and aging of bone marrow mesenchymal stem cells regulating ischemia-hypoxia microenvironment

背景:中药调控缺血缺氧微环境,提高干细胞生存率、分化率,对治疗缺血性心脑血管疾病有一定的价值和意义。目的:整理和剖析近年来中医药调控缺血缺氧微环境干预骨髓间充质干细胞增殖、分化、衰老、自噬的研究进展。方法:以“bone mesenchymal stem cells,ischemia-hypoxia microenvironment,proliferation,differentiation,aging”或“骨髓间充质干细胞,缺血缺氧,增殖,分化,衰老”为主题检索词,检索中国期刊全文数据库、PubMed、万方数据库中2002至2019年关于缺血缺氧微环境对骨髓间充质干细胞生存率及分化率影响的相关文章,选择55篇进行综述,其中中文文献22篇,英文文献33篇。结果与结论:缺血缺氧微环境是骨髓间充质干细胞存活率和分化率低的重要原因,基因修饰或用细胞分子和药物干预骨髓间充质干细胞存在诸多不良反应,成为现代医学需要攻克的难题。运用中药单体或活性成分联合RNA转录组学探索微环境与干细胞的内在联系,是提高干细胞生存能力的新思路。

BACKGROUND:Traditional Chinese medicine has certain value and significance in the treatment of ischemic cardiovascular and cerebrovascular diseases by regulating the ischemia-hypoxia microenvironment and improving the survival rate and differentiation rate of stem cells.OBJECTIVE:To sort out and analyze the research progress of traditional Chinese medicine on regulating ischemia-hypoxia microenvironment intervention on proliferation,differentiation,aging and autophagy of bone marrow mesenchymal stem cells in recent years.METHODS:The full-text database of Chinese journals,PubMed and Wanfang were retrieved with the keywords of“bone mesenchymal stem cells,ischemiahypoxia microenvironment,proliferation,differentiation,aging”in English or“bone marrow mesenchymal stem cells,ischemia and hypoxia,proliferation,differentiation,aging”in Chinese for articles regarding effects of ischemia and hypoxia microenvironment on survival rate and differentiation rate of bone marrow mesenchymal stem cells published from 2002 to 2019.Fifty-five articles were selected for review,including 22 Chinese articles and 33 English articles.RESULTS AND CONCLUSION:The ischemia-hypoxia microenvironment is the important reason for the low survival rate and differentiation rate of bone marrow mesenchymal stem cells.There are many adverse reactions in the intervention of bone marrow mesenchymal stem cells with gene modification or cell molecules and drugs,which have become difficult problems to be solved in modern medicine.Exploring the internal relationship between microenvironment and stem cells using single or active components of traditional Chinese medicine combined with RNA transcriptomics is a new way to improve the viability of stem cells.