响应面法提取栀子多糖及其活性研究

Optimization of extraction and the activity of polysaccharides from Gardenia by response surface methodology

优化了栀子多糖的提取工艺,并对栀子多糖的抗氧化能力及其对α-葡萄糖苷酶的抑制活性进行了测定。在单因素试验的基础上,选择了超声时间、超声功率、液料比为考察因素,以栀子多糖的提取率为响应值,用Box-Behnken试验进行了三因素三水平设计,通过响应面法来优化栀子多糖的提取工艺。结果表明,栀子多糖的最佳提取工艺为:提取时间为73 min,液料比为44∶1(mL∶g),功率为120 W,在此条件下,栀子多糖的提取率为(6.34±0.09)%。栀子多糖的抗氧化能力和对α-葡萄糖苷酶的抑制活性均小于同浓度的维生素C。栀子多糖对自由基清除能力的强弱顺序为:OH·>DPPH·>ABTS^+·>O^-2。

Extraction process of polysaccharides from Gardenia was optimized.The inhibition rate of polysaccharides and the scavenging activity from Gardenia were investigated as well.Based on single-factor tests,the three-factor and three-level experiments were designed through Box-Behnken experiments.Meanwhile ultrasonic time,ultrasonic power and liquid-solid ratio were chosen as affecting factors;Extraction ratio of Gardenia polysaccharides was set as response value.Results showed that the best extraction conditions of Gardenia polysaccharides were as follows:extraction time was 73 min,liquid-solid ratio was 44∶1(mL∶g),ultrasonic power was 120 W.The actual yield rate of Gardenia polysaccharides was(6.34±0.09)%.The antioxidant activity and the inhibitory activity of polysaccharides from Gardenia were less than those of vitamin C at the same concentration.The ability to scavenge free radicals with polysaccharides of Gardenia was presented in the following order:OH·>DPPH·>ABTS^+·>O^-2·.