灵芝多糖的抗氧化作用研究

Study on Antioxidation of Ganoderma Lucidum Polysaccharide

目的研究灵芝多糖的抗氧化作用,为灵芝多糖化妆品抗皮肤衰老功效提供科学依据。方法化学方法评价灵芝多糖的总还原能力、羟基自由基和DPPH自由基清除能力;检测灵芝多糖脂质体过氧化进程中过氧化物和丙二醛(MDA)的产量考察其抗脂质体氧化能力;体外细胞实验考察灵芝多糖对H2O2诱导氧化损伤的HaCaT细胞的保护作用;在体实验考察自制灵芝多糖乳液对D-半乳糖亚急性小鼠衰老模型皮肤中超氧化物歧化酶(SOD)活力、MDA和羟脯氨酸(Hyp)含量的影响。结果灵芝多糖的还原能力和清除2种自由基能力均呈浓度依赖性,而且它能抑制脂质过氧化物生成,60 h灵芝多糖脂质体产生的过氧化物为空白脂质体的九分之一,MDA产量为空白脂质体的三分之一。灵芝多糖在0.3125~1.25 mg/mL的范围内对H2O2氧化损伤的HaCaT细胞有保护作用(P<0.01),且保护作用随多糖浓度升高而增强。小鼠皮肤涂抹低剂量自制灵芝多糖乳液后,皮肤中SOD活力显著高于模型组(P<0.05);高剂量涂抹时,MDA含量显著下降,Hyp含量显著升高(P<0.05),自制灵芝多糖乳液抗氧化能力略优于市售乳液。结论多层面评价了灵芝多糖的抗氧化活性,初步研制出具有抗衰老功效的灵芝多糖乳液,为灵芝多糖抗衰老化妆品的开发提供研究基础。

OBJECTIVE To study the antioxidant activity of Ganoderma lucidum polysaccharides(GLP),and to provide scientific basis for its application of anti-aging cosmetics.METHODS Chemical method was used to evaluate the total reducing ability,hydroxy radical and DPPH free radical scavenging ability of GLP.The anti-lipid peroxidation ability of GLP was investigated with respect to the production of peroxid and malondialdehyde.The effects of GLP on the protective effect of H2O2-induced oxidative damage on HaCaT cells was investigated by in vitro cell experiments.The effects of lab-made GLP emulsion on superoxide dismutase(SOD)activity,malondialdehyde(MDA),and hydroxyproline(Hyp)contents in the skin of D-galactose subacute mouse aging model were investigated in vivo.RESULTS The reducing ability and the ability to scavenge two radicals above were concentration-dependent.At the same time,it showed that GLP could inhibit the production of lipid peroxidation products.At 60 h,the peroxide produced by GLP liposomes were one-ninth of that of blank liposomes,and the production of malondialdehyde were one-third of it.Furthermore,GLP had a protective effect on H2O2 induced oxidatively damaged HaCaT cells(P<0.01),and the protective effect was enhanced with increasing polysaccharide concentration.The results of animal experiments showed that the SOD activity in the skin was significantly higher than that in the model group(P<0.05)after applying low-dose homemade GLP emulsion to the skin of mice;when applied at high doses,the MDA content decreased significantly and the Hyp content increased significantly(P<0.05),the antioxidant capacity of lab-made GLP emulsion is slightly better than the commercially available emulsion.CONCLUSION The antioxidative activity of GLP was evaluated at multiple levels,and GLP emulsions with anti-aging effects were initially prepared,which provided a research basis for the development of GLP anti-aging cosmetics.