ICP患者血清疏水性胆汁酸与miR-18a水平的相关性研究

Study on the correlation between serum hydrophobic bile acid and the level of miR-18a in patients with ICP

目的探讨妊娠期肝内胆汁淤积症(ICP)患者血清miR-18a水平与血清疏水性胆汁酸的相关性。方法选取健康对照组、轻度ICP组、中度ICP组、重度ICP组孕妇各40例,分别采用实时定量RT-PCR法检测血清miR-18a水平,高效液相色谱串联质谱法检测疏水性胆汁酸脱氧胆酸、鹅脱氧胆酸、石胆酸、甘氨鹅脱氧胆酸水平;并与血清miR-18a水平进行相关性分析。结果相较于健康对照组,轻度、中度和重度ICP组患者血清miR-18a水平均明显降低,差异有统计学意义(P<0.05);相较于轻度ICP组,中度和重度ICP组患者血清miR-18a水平明显降低,差异有统计学意义(P<0.05);相较于中度ICP组,重度ICP组患者血清miR-18a水平明显降低,差异有统计学意义(P<0.05)。对照组孕妇血清miR-18a水平与疏水性胆汁酸水平无相关性(P>0.05);轻度、中度和重度ICP组患者血清miR-18a水平与疏水性胆汁酸水平呈负相关(r=-0.847^-0.531,P<0.05)。结论ICP患者血清miR-18a明显降低,随病情加重,miR-18a水平越低,miR-18a水平与血清疏水性胆汁酸水平呈负相关,可能在ICP的发生、发展中发挥重要作用。

Objective To investigate the correlation between serum level of miR-18a in patients with intrahepatic cholestasis of pregnancy(ICP)and serum hydrophobic bile acid.Methods 160 pregnant women were divided into health control group,mild ICP group,moderate ICP group and severe ICP group,40 cases in each group.The serum level of miR-18a was detected by real-time quantitative RT-PCR.The levels of deoxycholic acid,goose deoxycholic acid,cholic acid,glycine-bound goose deoxycholic acid were detected by high performance liquid chromatography tandem mass spectrometry,and the correlation with miR-18a were analyzed.Results Compared with the health control group,the serum levels of miR-18a in mild ICP,moderate ICP and severe ICP groups were decreased,and the differences were statistically significant(P<0.05).Compared with the mild ICP group,the serum levels of miR-18a in moderate ICP and severe ICP groups were decreased,and the differences were statistically significant(P<0.05).Compared with the moderate ICP group,the serum level of miR-18a in severe ICP group was decreased,and the difference was statistically significant(P<0.05).There was no correlation between the serum level of miR-18a and hydrophobic bile acid in the control group(P>0.05),there was a negative correlation between the serum level of miR-18a and hydrophobic bile acid in mild,moderate and severe ICP group(r=-0.847--0.531,P<0.05).Conclusion The serum level of miR-18a in ICP patients is significantly decreased.With the aggravation of the disease,the level of miR-18a decreased.There is a negative correlation between the level of miR-18a and the level of hydrophobic bile acid in serum.miR-18a may play an important role in the occurrence and development of ICP.