葛根对细菌性腹泻模型小鼠淋巴细胞水平和胃肠传输功能的影响研究

Effect of pueraria lobata on lymphocyte level and gastrointestinal transport function in rats with bacterial diarrhea

目的:探讨葛根对细菌性腹泻模型小鼠外周血浆Th1、Th2、Th17表达的影响,并观察其对胃肠传输功能的作用。方法:制备胃肠感染模型小鼠20只,随机分为模型组10只及干预组10只,干预组利用葛根水溶性生物碱灌胃,连续7 d;再将10只正常小鼠设为空白组。比较各组腹泻次数、外周血中Th1、Th2、Th17、血细胞表达水平,IL-2、IFN-γ、IL-4、IL-10、IL-17A、胃动素、血管活性肠肽的表达水平以及小鼠结肠传输时间。结果:造模后小鼠腹泻次数明显增加,造模后小鼠Th1、Th2细胞百分比、IL-2、IFN-γ、IL-4、IL-10、胃平滑肌层Obestatin及其受体GPR39、结肠传输时间明显下降,白细胞数量、中性粒细胞百分比、淋巴细胞百分比、Th17细胞百分比、IL-17A升高,与空白组比较差异均有统计学意义(P<0.05),经过葛根干预后上述指标明显改善,与模型组比较差异有统计学意义(P<0.05)。结论:葛根可明显改善胃肠感染,其作用机制与介导机体免疫反应有关。

Objective:To explore the effect of Pueraria lobata on the expression of Th1,Th2 and Th17 in peripheral plasma of mice with bacterial diarrhea,and to observe its effect on gastrointestinal transport function.Methods:Twenty mice with gastrointestinal infection were randomly divided into model group(n=10)and intervention group(n=10).The intervention group was fed with Pueraria water-soluble alkaloids for 7 days,and 10 normal mice were set as blank group.The times of diarrhea,the expression levels of Th1,Th2,Th17 and blood cells in peripheral blood,the expression levels of IL-2,IFN-γ,IL-4,IL-10,IL-17A,motilin,vasoactive intestinal peptide and colonic transit time in mice were compared.Results:After the establishment of the model,the number of diarrhea in mice increased significantly,and the percentage of Th1,Th2 cells,IL-2,IFN-γ,IL-4,IL-10,obestatin and its receptor GPR39 in gastric smooth muscle layer and colonic transit time decreased significantly,while the number of white blood cells,neutrophil percentage,lymphocyte percentage,Th17 cell percentage and IL-17A increased significantly compared with the blank group.After the intervention of Pueraria lobata,the above indexes were significantly improved compared with the model group(P<0.05).Conclusion:Pueraria lobata can significantly improve gastrointestinal infection,and its mechanism is related to mediating immune response.