辣椒不同发育时期果实着色的转录组分析

Transcriptome Analysis of Fruits Coloration at Different Developmental Stages in Capsicum annum

为研究辣椒果实不同发育时期着色的基因表达差异,探究参与或调控辣椒果实类胡萝卜素合成的基因或转录因子。本研究采用RNA-Seq技术对辣椒果实青果期(G)、转色期(B1,果实表面30%左右变色)、转色期(B2,果实表面50%左右变色)和成熟期(R)的辣椒果实进行了转录组测序分析。结果表明,G-vs-B1、B1-vs-B2和B2-vs-R上调和下调的差异表达基因(DEGs)分别为924个和2188个;DEGs经GO富集分析主要集中在生物调控、应激反应、信号、代谢过程和生长等的生物过程;DEGs经KEGG富集分析主要富集在类胡萝卜素生物合成和淀粉、蔗糖代谢等信号通路,DEGs共注释到3079个转录因子,包括AP2、MYB、MADS和ERF等类型,转录因子AP2在调控植物类胡萝卜素的生物合成方面已得到证实,但在辣椒果实着色过程中的作用还有待研究。本研究为进一步挖掘辣椒果实着色过程中的关键调控因子,解析调控类胡萝卜素生物合成提供理论基础。

In order to study the gene expression difference of pepper fruit coloring at different development stages,genes or transcriptional factors involved in or regulating carotenoid synthesis in pepper fruits were excavated.In this study,RNA-seq technology was used to analyze the changes of pepper fruits during green stage(G),breaking stage(B1,about 30%of fruit surface discoloration),breaking stage(B2,about 50%of fruit surface discoloration),and red stage(R).The results showed that,924 genes were up-regulated and 2188 genes were down-regulated during G-vs-B1,B1-vs-B2 and B2-vs-R.GO enrichment analysis showed that DEGs were mainly involved in biological processes such as biological regulation,stress response,signaling,metabolic processes,growth,KEGG enrichment analysis showed that DEGs were mainly involved in in signal pathways like carotenoid biosynthesis and starch and sucrose metabolism.DEGs were annotated to a total of 3079 transcription factors,including AP2,MYB,MADS and ERF.The role of transcription factor AP2 in the regulation of carotenoid biosynthesis in plants has been confirmed,but the role of AP2 in the process of capsicum fruit coloring remains to be studied.This study provides a theoretical basis for further excavating key regulatory factors in the coloring process of pepper fruits and analyzing and regulating carotenoid biosynthesis.