禾谷镰刀菌MYB转录因子生物信息学分析

Bioinformatics Analysis of MYB Transcription Factors in Fusarium graminearum

本研究利用生物信息学方法对真菌转录因子数据库收录的17条禾谷镰刀菌MYB转录因子序列进行了分析。基因序列和蛋白理化性质分析发现,禾谷镰刀菌17个MYB转录因子在核酸长度、蛋白大小、理论等电点、原子总数以及不稳定系数方面均存在差异;基序分析和蛋白二级、三级结构预测发现,这些转录因子包含2条特征基序,蛋白空间结构多样且均包含MYB转录因子特征结构(螺旋-转角-螺旋(HTH));系统进化树分析表明,17个禾谷镰刀菌MYB转录因子与12个已报道的其他物种MYB转录因子进化出两个大的分支,其中Fg MYB03、Fg MYB04、Fg MYB05、Fg MYB06、Fg MYB10、Fg MYB12和Fg MYB13与已知功能的动物、植物和真菌MYB转录因子c-MYB、ATMYB2、MoMyb1、FlbD和Pol5等具有较近的亲缘关系,推测它们具有类似的生物学功能。本研究结果为进一步研究禾谷镰刀菌MYB转录因子的生物学功能以及其他真菌MYB转录因子的家族特征提供了依据。

The bioinformatics method was used to analyze the 17 Fusarium graminearum MYB transcription factors(TFs)included in the fungal transcription factor database.Analysis of gene sequence and protein physical and chemical properties showed that the 17 F.graminearum MYB TFs differ in gene length,protein size,theoretical isoelectric point,total number of atoms,and instability index;Motif analysis and protein secondary and tertiary structure prediction found that these transcription factors contain 2 characteristic motifs,and that the protein structures are diverse,but all contain the MYB TF characteristic structure(helix-turn-helix(HTH));Phylogenetic tree analysis showed that 17 F.graminearum MYB TFs and 12 reported MYB TF3 from other species have evolved into two large clades.Among them,FgMYB03,FgMYB04,FgMYB05,FgMYB06,FgMYB10,FgMYB12 and FgMYB13 have close relationship with known animal,plant and fungal MYB TFs including c-MYB,ATMYB2,MoMyb1,FlbD and Pol5,which implied that they possibly share similar biological functions.The results provide a reference for further research on the biological function of F.graminearum MYB TFs and the feature of other fungal MYB TF family.