盐胁迫培养下季也蒙毕赤酵母的转录组学差异分析

Transcriptomic difference analysis of Meyerozyma guilliermondii in response to salt stress

在本实验室从海南省西沙群岛海沙里分离出1株季也蒙毕赤酵母(Meyerozyma guilliermondii),并证实该酵母菌株有较好的耐盐性,在12%NaCl胁迫培养条件下仍可生长的基础上,本文通过Illumina HiSeqTM高通量测序技术对经盐胁迫培养与非盐胁迫培养24 h的M. guilliermondii进行转录组测序比较。结果表明:2组样品共有1 027个显著性差异表达基因,其中458个基因表达上调,569个基因表达下调。通过基因本体(gene ontology, GO)功能注释发现,经盐胁迫处理后M. guilliermondii的差异表达基因主要富集在生物过程分类中,其中核苷酸代谢、糖代谢及辅酶代谢基因产生较大差异。对差异基因进行京都基因与基因组百科全书数据库(Kyoto Encyclopedia of Genes and Genomes, KEGG)通路富集分析,发现大部分富集通路都与细胞分裂和代谢有关,与GO富集结果相对应。上述结果可以为盐胁迫培养对M. guilliermondii的生长影响及进一步的生物学探究提供科学依据。

Previous work proved that a strain of Meyerozyma guilliermondii isolated from the Xisha islands of Hainan Province had good salt-tolerant ability which could grow under 12%NaCl stress culture condition.Based on the above results,the transcriptome sequencing of the strain cultured under salt stress and non-salt stress for24 h was constructed by Illumina HiSeqTMin this study.The results were as follows:the two samples yielded1027 significantly differential expression genes,of which 458 genes were up-regulated and 569 genes were down-regulated.According to the gene ontology(GO)functional annotations,the differential expression genes of M.guilliermondii treated with salt stress were mainly concentrated on the classification of biological process,in which nucleotide metabolism,sugar metabolism and coenzyme metabolism genes were greatly different.Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis of the differential expression genes showed that the most of the enrichment pathways were related to cell division and metabolism,which were corresponding to the GO enrichment results.The above results can provide scientific basis for further biological research on the effect of the growth of M.guilliermondii under salt stress.