Fmr1基因敲除型小鼠肠道菌群结构分析

Analysis of intestinal flora composition in Fmr1 knockout mice

目的探讨Fmr1基因敲除型小鼠粪便肠道细菌群落的结构及多样性。方法利用16S rRNA高通量测序技术对10份小鼠粪便样本进行测序分析并进行生物学分析。结果两组样本测序共获得高质量序列325 280条,核心菌门为拟杆菌门、厚壁菌门、变形菌门。Anosim分析表明基因敲除(KO)组与正常野生型(WT)组小鼠两组间具有显著差异,组内样品重复性满足数据分析要求,Adonis分析表明本次检验可信度高(P<0.05)。LDA值展现KO组与WT组小鼠肠道微生态菌群有明显差异,基于Unifrac距离,Amova分析表明KO和WT组组间差异显著(P<0.05)。结论 KO组与WT组小鼠肠道微生物群落的结构及多样性存在显著差异且具有统计学意义,提示孤独症与肠道微生态系统密切相关,肠道微生态系统可能通过微生物代谢的间接作用影响孤独症的发生发展,但具体的作用机制仍需进一步的研究。

Objective To explore the structure and diversity of fecal bacterial community in Fmr1 knockout mice.Methods 10 mouse feces samples were sequenced and analyzed by 16S rRNA highthroughput sequencing.Results A total of 325280 high quality sequences were obtained from the two groups of samples.And 3 core bacteria were Bacteroidetes,Firmicutes and Proteobacteria.The analysis of Anosim showed that there were significant differences between the knockout(KO)and the wild-type(WT)groups.The repeatability of the samples within the group meets the data analysis requirements.The analysis of Adonis showed that the reliability of this test was high(P<0.05).LDA showed significant differences in intestinal microflora between KO and WT groups.Based on the distance of Unifrac,the analysis of Amova showed significant differences between KO and WT groups(P<0.05).Conclusions The intestinal microbial community structure and diversity of KO and WT groups exists significant differences,indicating that autism is closely related to the intestinal micro ecology system.The intestinal microecology system may affect the development of autism by microbial metabolism indirectly,but the exact mechanism still needs further research.