引火汤改善长期OVX小鼠焦虑样行为的机制研究

Mechanism of Yinhuo Decoction on Improving Anxiety-Like Behavior in Long-Term OVX Mice

目的:探讨引火汤治疗长期双侧卵巢摘除术(OVX)小鼠的焦虑样行为的机制。方法:将40只ICR小鼠随机分为假手术组(Sham组)、模型组(OVX组)、雌二醇组(E 2组)和引火汤组(YHT),采用双侧卵巢摘除联合单频超声刺激复制长期OVX焦虑模型,采用明暗箱(LDB)和高架十字迷宫(EPM)观察小鼠的焦虑样行为,检测各组小鼠脑内雌激素水平,测量体质量、子宫系数,HE染色观察子宫形态,尼氏染色观察海马神经元状态。结果:与OVX组相比,YHT组小鼠焦虑样行为显著改善,脑内雌激素水平显著增加,海马神经元形态完好,子宫形态未见改变。结论:引火汤能改善长期OVX小鼠的焦虑样行为,其作用机制可能是发挥雌激素效应并对ERβ有选择性激活作用。

Objective:To investigate the mechanism of Yinhuo Decoction in treating long-term anxiety-like behavior in OVX mice.Methods:40 ICR mice were randomly divided into the sham operation group,the model group(OVX),estradiol(E 2)group and Yinhuo Decoction group.The bilateral ovarian ablation combined with single-frequency ultrasound stimulation was used for long-term OVX replication.LDB and EPM tests were used to detect anxiety-like behaviors.The level of estrogen in the brain was measured.The body weight and uterus coefficient were measured.The morphology of the uterus was observed by HE staining.The hippocampal neuron status was observed by Nissl staining.Results:Compared to the OVX group,the anxiety-like behavior of Yinhuo Decoction group was significantly improved,the level of estrogen in the brain was significantly increased,the morphology of the hippocampal neurons was intact,and the morphology of the uterus was unchanged.Conclusion:Yinhuo Decoction can improve the anxiety-like behavior of long-term OVX mice.The mechanism of action may be related to exerting estrogenic effects and selectively activating ERβ.