HS-SPME-GC-MS分析大青叶和板蓝根中挥发性成分

Analysis of volatile components in folium isatidis and radix isatidis by HS-SPME-GC-MS

采用顶空固相微萃取法(HS-SPME)与气质联用技术(GC-MS)相结合对大青叶和板蓝根中挥发性成分进行比较分析,利用面积归一化法计算各成分的相对百分含量。结果表明,从大青叶中分离出38个峰,鉴定出31种成分,占挥发性成分的81.58%;从板蓝根中分离出35个峰,鉴定出28种成分,占挥发性成分的80.00%;两者共有成分有16种,分别占大青叶和板蓝根挥发性成分总量的48.16%和48.40%。大青叶和板蓝根中挥发性成分和含量有一定的差异,同时二者又有一部分相同的药理作用。

The volatile components of folium isatidis and radix isatidis were analyzed and compared by headspace solid phase microextraction(HS-SPME)combined with GC-MS.The relative percentage of each component was calculated by area normalization method.Results showed that 38 peaks were isolated from folium isatidis and 31 components were identified,accounting for 81.58% of the volatile components.35 peaks were isolated from radix isatidis and 28 components were identified,accounting for 80.00% of the volatile components.There are 16 kinds of volatile components in the two,which account for 48.16% and 48.40% of the total volatile components of folium isatidis and radix isatidis,respectively.There were some differences in the volatile components and content of folium isatidis and radix isatidis,and they have some of the same pharmacological effects.