支气管肺癌患者血清和癌组织中瘦素水平的表达及临床意义

Expression and Clinical Significance of Leptin in Serum and Cancer Tissues of Patients with Bronchial Lung Cancer

目的研究瘦素(Leptin)在支气管肺癌患者血清和肺癌组织中的表达及临床意义。方法118例肺癌患者(肺癌组)和24例正常人(对照组)的血清Leptin水平采用酶联免疫法(ELISA)测定,118例手术切除的肺癌标本及24例正常肺组织中的Leptin表达情况采用免疫组织化学SP法检测。结果肺癌组患者血清瘦素(3.6±1.5μg/L)水平显著高于对照组(1.6±0.7μg/L),差异有统计学意义(t=3.485,P=0.002)。肺癌患者肺组织中瘦素表达的阳性率为75.42%(89/118),正常肺组织瘦素表达的阳性率为25.0%(6/24),两者比较差异有统计学意义(P<0.05)。男性肺癌患者瘦素的表达阳性率为95.0%(57/60),女性为55.17%(32/58),两者相比差异有统计学意义(t=25.237,P=0.000)。支气管肺癌患者的年龄、病理类型对Leptin表达阳性率的影响无显著性差异(χ2=0.095,0.013,均P>0.05)。肺癌患者的组织分化程度、临床分期、有无淋巴结转移对Leptin表达阳性率的影响有显著性统计学差异(χ2=7.249,P<0.05)。吸烟指数≥400的肺癌患者瘦素表达阳性率68.54%(61/89),吸烟指数<400的患者为31.46%(28/89),两者相比差异有统计学意义(χ2=12.44,P=0.000)。结论肺癌患者的血清Leptin水平显著高于正常人的血清Leptin水平。支气管肺癌组织中Leptin蛋白高表达,Leptin的表达与肺癌患者的年龄、病理类型无关,与肺癌患者性别、组织的分化程度、临床分期、有无淋巴结转移、吸烟指数密切相关。

Objective To study the expression and clinical significance of leptin in the serum and lung cancer tissues of patients with bronchial lung cancer.Methods Serum leptin levels in 118 patients with lung cancer(lung cancer group)and 24 normal persons(control group)were measured by radioimmunoassay(ELISA).The expression of leptin in 118 paraffin specimens of surgically removed lung cancer and 24 normal lung tissues Immunohistochemical SP method.Results The level of serum leptin(3.6±1.5μg/L)in patients with lung cancer was significantly higher than that in control group(1.6±0.7μg/L),and the difference was statistically significant(t=3.485,P=0.002).The positive rate of leptin expression in lung tissue of patients with lung cancer was 75.42%(89/118)and 25.0%(6/24)in normal lung tissue,and the difference was statistically significant(P<0.05).The positive rate of leptin expression in male lung cancer patients was 95.0%(57/60)and 55.17%(32/58)in females,and there was significant difference between the two groups(t=25.237,P=0.000).The age and pathological type of patients with bronchial lung cancer had no significant statistical difference in the positive rate of Leptin expression(χ2=0.095,0.013,all P>0.05).The degree of tissue differentiation,clinical stage,and presence or absence of lymph node metastasis in lung cancer patients had significant statistics on the positive rate of leptin expression academic difference(χ2=7.249,P<0.05).The positive rate of leptin expression in lung cancer patients with smoking index≥400 was 68.54%(61/89),and that in patients with smoking index<400 was 31.46%(28/89).The difference between the two was statistically significant(χ2=12.44,P=0.000).Conclusion The level of serum leptin in patients with lung cancer was significantly higher than that in normal people.Leptin protein was highly expressed in bronchial lung cancer tissues.The expression of leptin had nothing to do with the age and pathological type of lung cancer patients,and was closely related to gender,tissue differentiation degree,clinical stage,lymph node metastasis,and smoking index of lung cancer patients.