新型冠状病毒肺炎患者血清SARS-CoV-2抗体的检测分析

Preliminary Analysis of Serum Antibodies Against SARS-CoV-2 in Patient with Coronavirus Disease 2019

目的分析新型冠状病毒肺炎(corona virus disease 2019,COVID-19)患者血清新型冠状病毒(severe acute respiratory syndrome coronavirus 2,SARS-CoV-2)IgM和IgG抗体的变化规律及临床应用。方法收集2020年1~2月初温州医科大学附属乐清医院收治的28例SARS-CoV-2核酸检测阳性的COVID-19患者作为病例组,排除COVID-19的30例其他疾病患者作为对照组。利用全自动化学发光免疫分析技术检测所有研究对象的血清SARS-CoV-2 IgM和IgG,分析SARS-CoV-2抗体的变化规律。结果SARS-CoV-2 IgM,发病第1~6天均阴性,发病第7~10天,2例患者阳性,发病第18天后均转阳性,一个月左右达峰值,2例患者分别在第49天和第55天出现9.80AU/ml(阴性)和10.79AU/ml(弱阳性)。SARS-CoV-2 IgG,发病第1~6天均阴性,发病第8天后均阳性。结论发病10天内SARS-CoV-2 IgM阴性的患者不能排除SARS-CoV-2感染,发病超过一周或多次核酸检测阴性的疑似患者检测SARSCoV-2 IgM和IgG具有重要意义。

Objective To explore the dynamic characteristics of serum IgM and IgG antibodies against SARS-CoV-2 in COVID-19 patient and their clinical application.Methods A total of 28 patients with COVID-19 and 30 patients without COVID-19 were involved in this study from January to February 2020 in Yueqing Hospital Affiliated to Wenzhou Medical University.The levels of serum IgM and IgG were measured by automatic chemiluminescence immunoassay analyzer,and the dynamic characteristics of SARS-CoV-2 antibodies was analyzed.Results On the 1st-6th day,SARS-CoV-2 IgM were negative,on the 7th-10th day,two COVID-19 patients’IgM were positive,and IgM were positive after eighteen days later.SARSCoV-2 IgM reached the peak in one month.On the 49th and 55th day,one COVID-19 patient’s IgM was 9.80AU/ml(negative)and the other was 10.79AU/ml(weakly positive).On the 1st-6th day,SARS-CoV-2 IgG were negative,IgG turned positive in one week.Conclusion It is suggested that infection can’t be excluded with negative IgM of SARS-CoV-2 within 10 days after initial onset of symptoms,and the IgM and IgG can be used for COVID-19 screening in suspected patients with negative nucleic acid detection for many times or the patients with symptom more than one week.