不同酶处理对再造烟叶梗膏的提质效果

Quality Improvement of Reconstituted Tobacco Stem Extract Treated by Different Enzymes

为梗膏在再造烟叶生产上的提质增效提供技术支撑,通过设置蛋白酶、木瓜蛋白酶和多酚氧化酶不同酶处理梗膏,对不同酶处理梗膏进行致香成分分析,并对不同酶处理梗膏制备成再造烟叶产品进行有害成分分析和感官质量评价。结果表明:在醇类、醛类和酮类等8个大类致香成分中,除酯类致香成分含量木瓜蛋白酶-酸性蛋白酶处理梗膏较未经过酶处理的梗膏有所降低外,其余类别致香成分含量木瓜蛋白酶-酸性蛋白酶和木瓜蛋白酶-多酚氧化酶处理梗膏均较未经过酶处理梗膏增加;木瓜蛋白酶-酸性蛋白酶和木瓜蛋白酶-多酚氧化酶处理梗膏再造烟叶产品感官评价总分均高于未经过酶处理的梗膏,分别提高13.38%和21.83%;危害性指数,未经过酶处理梗膏再造烟叶产品最高,为5.19,木瓜蛋白酶-多酚氧化酶处理梗膏再造烟叶产品最低,为3.29,木瓜蛋白酶-酸性蛋白酶和木瓜蛋白酶-多酚氧化酶处理梗膏再造烟叶产品较未经过酶处理梗膏再造烟叶产品分别下降17.92%和36.61%。不同酶处理梗膏均能有效改善再造烟叶产品的感官品质,降低木质杂气和刺激,从而提高梗膏的利用率。

The aroma components of stem extract under different enzyme treatments(acid protease,papain and polyphenol oxidase)are analyzed,and the harmful components and sensory quality of reconstituted tobacco product prepared with stem extract treated by different enzymes are analyzed and evaluated to provide the technological support for improving quality and efficiency of stem extract in production of reconstituted tobacco.Result:The content of esters aromatic components in tobacco stem extract under papain-acid protease treatment decreases compared with CK(tobacco stem extract without enzyme treatment),and other seven aromatic components(alcohols,aldehydes,ketones,etc.)in tobacco stem extract under papain-acid protease treatment and papain-polyphenol oxidase treatment increase compared with CK.The sensory evaluation score of reconstituted tobacco product from stem extract under papain-acid protease treatment and papain-polyphenol oxidase treatment both increases by 13.38%and 21.83%compared with CK.The hazardous index in reconstituted tobacco product from stem extract without enzyme treatment is 5.19(the highest),and the hazardous index in reconstituted tobacco product from stem extract under papain-polyphenol oxidase treatment is 3.29(the lowest).The hazardous indexes in reconstituted tobacco product from stem extract under papain-acid protease treatment and papain-polyphenol oxidase treatment decreases by 17.92%and 36.61%compared with CK respectively.The stem extract under different enzyme treatments can improve sensory quality and reduce woody impurities and irritation of reconstituted tobacco product,and thereby increasing utilization efficiency of tobacco stem extract.