谷子bZIP转录因子家族的全基因组鉴定与生物信息学分析

Genome-wide Identification and Bioinformatics Analysis of Setaria italica bZIP Transcription Factor Family

谷子是我国重要的杂粮作物之一,其籽粒营养价值高、可药用,茎秆可以作为饲料。bZIP基因家族是植物中家族成员数量最多的转录因子家族之一,其调节包括病原防御、光反应和胁迫信号、种子成熟和花发育等过程。利用谷子的全基因组数据库,通过多种生物信息软件及在线网站对谷子bZIP转录因子家族进行全基因组鉴定,并分析其系统进化、基因结构、蛋白质理化性质、保守基序、启动子顺式作用元件和表达量情况。结果表明,谷子bZIP转录因子家族共有85个基因;亲缘关系较近的谷子bZIP基因的基因结构较为相似;所有bZIP转录因子蛋白质都有模体motif1,motif1在蛋白质序列中最为保守,其中的碱性氨基酸R以及L最保守;bZIP转录因子家族基因的启动子顺式作用元件种类较多,包括ABRE、ARE、CAT-box、G-box等元件,其参与调控胁迫响应、生长发育等过程。基因表达分析结果表明,亲缘关系较近的家族成员具有相似的表达特异性,SibZIP转录因子在根、花和叶片中均有成员表达;SibZIP11、SibZIP12和SibZIP72等在干旱处理的根中表达量较高。谷子bZIP转录因子家族分析结果可为进一步研究谷子bZIP基因功能和分子机制奠定理论基础。

Setaria italica is one of the important multigrain crops in China,and its seeds have high nutritional value and can be used for medicine,its stalks can be used as fodder.The bZIP(basic leucine zipper)gene family is one of the largest families of transcription factors in plants,regulating processes including pathogen defense,light and stress signals,seed maturation and flower development.In this study,the whole genome database of foxtail millet was used to identify the whole genome of millet bZIP transcription factor family,then analyze the phylogenetic relationship,gene structure,physical and chemical properties of proteins,conserved motif,promoter cis-acting element and expression level of foxtail millet bZIP gene family members through a variety of biological information software and online websites.The results showed that there were 85 genes in the bZIP transcription factor family of foxtail millet.The genetic structure of closely related foxtail millet bZIP gene was similar.All bZIP transcription factor proteins had motif1,and motif1 was the most conserved in the protein sequence,among which the basic amino acids R and L were the most conserved.There were many types of cis-acting elements in the promoter of bZIP transcription factor family genes,including ABRE,ARE,CAT-box,G-box,and so on,which participated in the regulation of stress response,growth and development.The gene expression analysis results indicated that members of closely related families had similar expression specificity,and SibZIP transcription factor was expressed in roots,flowers and leaves.SibZIP11,SibZIP12 and SibZIP72 were highly expressed in drought-treated roots.The analysis results of foxtail millet bZIP transcription factor family will lay a theoretical foundation for the further study of foxtail millet bZIP gene function and molecular mechanism.