龙脷叶转录组分析及黄酮类生物合成相关基因的挖掘

Analysis of Transcriptome and Exploration of Genes Related to Flavonoid Biosynthesis in Sauropus spatulifolius Beille

【目的】获得龙脷叶(Sauropus spatulifolius Beille)转录组学信息特征及黄酮类生物合成通路基因。【方法】采用高通量测序平台Illumina HiSeqTM 4000对龙脷叶进行转录组测序,通过Trinity软件de novo组装获得unigene,并基于序列同源性对unigene进行功能注释和代谢通路分析。【结果】测序数据经过质控后共获得88396692个高质量的reads,通过de novo组装获得46600个unigene,N50长度为1441 bp,平均长度877 bp。其中34188个(73.36%)unigene在NR、SwissProt、KOG、GO、KEGG数据库中得到注释。其中KEGG数据库注释到6902个unigene,涉及132条代谢通路。在龙脷叶中我们共鉴定到56个unigene涉及类黄酮生物合成、9个unigene参与黄酮和黄酮醇生物合成,2个unigene参与异黄酮生物合成。同时还鉴定到1256个转录因子(transcription factors,TFs)和3842个植物抗性基因(R基因)。MISA分析发现46600个unigene包含3356个简单重复序列(simple sequence repeats,SSRs)。【结论】利用高通量测序技术和生物信息分析获得了龙脷叶的转录组信息特征,为后期开展功能基因鉴定、解析黄酮类化合物次生代谢途径及其调控机制奠定研究基础。

【Objective】The study was conducted to obtain the transcriptomic characteristics and to identify the synthesis pathway genes related to flavonoid biosynthesis in Sauropus spatulifolius Beille.【Method】The high-throughput sequencing platform Illumina HiSeqTM 4000 was used to perform transcriptome sequencing and the unigene was assembled through de novo assembly by Trinity software.Then the functional annotation and metabolic pathway analysis of these unigenes were performed based on sequence homology.【Result】After quality control of sequencing data,a total of 88396692 highquality reads were obtained,and 46600 unigenes were obtained through de novo assembly.The N50 length was 1441 bp,with an average length of 877 bp.A total of 34188(73.36%)unigenes were annotated in NR,SwissProt,KOG,GO and KEGG databases.Among them,6902 unigenes were annotated in the KEGG database,involving 132 metabolic pathways.It was identified that 56 unigenes were involved in flavonoid biosynthesis,9 unigenes involved in flavone and flavonol biosynthesis,and 2 unigenes involved in isoflavonoid biosynthesis.1256 transcription factors(TFs)and 3842 plant resistance genes(R genes)were also identified.MISA analysis showed that 46600 unigenes contained 3356 simple sequence repeats(SSRs).【Conclusion】Transcriptome information characteristics of S.spatulifolius Beille were obtained by the high-throughput sequencing technology and biological information analysis,which provided a research basis for future researches to identify functional genes,flavonoid secondary metabolic pathways and their regulatory mechanisms.