黄瓜花叶病毒在洛阳地区的发生及亚组鉴定

Occurrence and Subgroup Identification of Cucumber mosaic virus in Luoyang Area

【目的】明确黄瓜花叶病毒(Cucumber mosaic virus,CMV)在洛阳地区主要蔬菜作物上的发生情况及其亚组类型,为该地区蔬菜作物上CMV的田间诊断和预防监测,防止CMV在该地区的大规模暴发等提供重要的理论依据。【方法】对采自洛阳地区的茄科、葫芦科和豆科等疑似病毒病蔬菜作物进行CMV的RT-PCR检测鉴定,利用相关分子生物学软件对获得的CMV CP基因进行序列分析,并构建系统进化树。【结果】86份疑似病毒病蔬菜样品中共检出CMV样品13份,检出率为15.12%,在番茄、辣椒、菜豆和花生上均有发生,该4个CMV CP基因核苷酸同源性为90.8%~98.8%,与已报道的CMV亚组Ⅰ和亚组Ⅱ分离物构建系统进化树发现该4种作物上的CMV分离物均为亚组Ⅰ病毒。【结论】CMV在洛阳地区茄科和豆科蔬菜作物上均有发生,且CMVⅡ可能为侵染的优势种病毒,应提前在作物种植早期做好蚜虫防控工作,并应在相关作物上积极开展CMVⅠ的抗病育种工作。

【Objective】The study was to identify the occurrence and subgroup types of Cucumber mosaic virus(CMV)on major vegetables in Luoyang City,and provide an important theoretical basis for the field diagnosis and prevention and monitoring to prevent the large-scale outbreak of CMV in this area.【Method】Samples with suspected virus symptoms collected from the Solanaceae,Cucurbitaceae and Leguminosae vegetables in Luoyang City,and were detected by RT-PCR with CMV primers,the obtained CMV CP gene sequences were analyzed by relevant biological software and the phylogenetic tree was constructed.【Result】Among 86 vegetable samples with suspected virus,CMV was detected in 13 samples from tomato,pepper,kidney bean and peanut,with a detection rate of 15.12%.The nucleotide homology of 4 CMV CP genes was 90.8%-98.8%,and it was found that the CMV isolates on these 4 crops belonged to CMV subgroupⅠstrains according to the phylogenetic tree construction of isolates in CMV subgroupⅠand CMV subgroupⅡ.【Conclusion】CMV occurred in Solanaceae and Leguminosae vegetables in Luoyang area,and CMVⅠmay be the dominant virus,indicating that aphid prevention and control should be done in advance at the early stage of crop planting,and CMVⅠdisease resistance bree ding should be actively carried out on relevant crops.