荧光定量RT-PCR快速检测乙型流感病毒核酸的价值分析

Value analysis of fluorescence quantitative RT-PCR for rapid detection of influenza B virus nucleic acid

目的分析用荧光定量逆转录-聚合酶链式反应(RT-PCR)快速检测乙型流感病毒核酸的临床价值。方法 104例乙型流感样患者作为研究对象,所有病例均采集咽拭子,均符合乙型流感的诊断标准。根据对乙型流感病毒检测方式的不同分为对照组和观察组,每组52例。对照组采用免疫学检测,观察组采用荧光定量RT-PCR检测。对比两组的检测敏感性,检测时间。结果观察组的检测敏感性为98.08%,高于对照组的82.69%,差异具有统计学意义(P<0.05)。观察组的检测时间为(11.2±2.1)min,短于对照组的(17.5±2.4)min,差异具有统计学意义(P<0.05)。结论荧光定量RT-PCR检测乙型流感病毒核酸可以明显提高对乙型流感病毒的检测敏感性,且检测时间短于免疫学检测,值得临床大力推广。

Objective To analyze the clinical value of fluorescence quantitative reverse transcriptasepolymerase chain reaction(RT-PCR)for rapid detection of influenza B virus nucleic acid.Methods 104 patients with influenza-like illness as the research subjects,all cases were collected throat swabs,all met the diagnostic criteria of influenza B.Patients were divided into control group and observation group according to the different methods of testing for influenza B virus,with 52 cases in each group.The control group was tested by immunology,and the observation group was tested by fluorescence quantitative RT-PCR.The detection sensitivity and detection time of the two groups were compared.Results The detection sensitivity of the observation group was 98.08%,which was higher than that of the control group 82.69%,and the difference was statistically significant(P<0.05).The detection time of the observation group was(11.2±2.1)min,which was shorter than that of the control group(17.5±2.4)min,and the difference was statistically significant(P<0.05).Conclusion Fluorescence quantitative RT-PCR detection of influenza B virus nucleic acid can significantly improve the sensitivity of influenza B virus detection,and the detection time is shorter than immunological detection,which is worthy of clinical promotion.