黔产大接骨丹药材不同用药部位中紫丁香苷和总黄酮含量的经时变化特征分析

Analysis of Ongoing Change Characteristics of the Contents of Syringin and Total Flavonoids in Different Medicinal Parts of Toricellia angulata from Guizhou

目的:研究黔产大接骨丹药材不同用药部位(根皮、树皮、叶)中紫丁香苷和总黄酮含量的经时变化特征,为该药材的开发和应用提供参考。方法:分别以不同采收期(1-12月)大接骨丹药材的根皮、树皮和叶部位为样品。采用高效液相色谱法测定其中紫丁香苷含量,色谱柱为Agela Promosil C18、流动相为0.5%磷酸溶液-乙腈(梯度洗脱)、流速为1.0 mL/min、检测波长为210 nm、柱温为35℃、进样量为5μL。采用紫外-可见分光光度法测定其中总黄酮含量,检测波长为510 nm。结果:紫丁香苷、总黄酮的质量浓度线性范围分别为0.0959~1.1508 mg/mL(r=0.9996)、0.0722~1.0830 mg/mL(r=0.9999);精密度、稳定性、重复性试验的RSD均小于3%(n=6);平均加样回收率分别为101.74%(RSD=2.36%,n=6)、99.63%(RSD=2.19%,n=6)。不同采收期内,大接骨丹药材根皮、树皮、叶中紫丁香苷含量分别在8、5、9月最高,总黄酮含量分别在2、12、9月最高。紫丁香苷含量在3个药用部位的大小依次为树皮>根皮>叶,其中树皮部位中紫丁香苷含量普遍较高;总黄酮含量在3个药用部位的大小依次为根皮>树皮>叶,且3个用药部位中总黄酮含量均普遍较低。根皮部位中总黄酮含量在当年2月最高,且当月根皮部位中紫丁香苷含量仅次于当年8月;树皮部位中紫丁香苷含量在5月最高,且当月树皮部位中总黄酮含量仅次于当年10、12月;叶部位中总黄酮含量与紫丁香苷含量均在当年9月最高。结论:建议大接骨丹药材根皮部位在2月采收为宜,树皮部位在5月采收为宜,叶部位在9月采收为宜。

OBJECTIVE:To study ongoing change characteristics of the contents of syringin and total flavonoids in different medicinal parts(root bark,tree bark,leaf)of Toricellia angulata from Guizhou,and to provide reference for the development and application of T.angulata.METHODS:The root bark,tree bark and leaf parts of T.angulata during different harvesting periods(Jan.-Dec.)were taken as the research samples.The content of syringin was determined by HPLC.The determination was performed on Agela Promosil C18 column with mobile phase consisted of 0.5%phosphoric acid solution-acetonitrile(gradient elution)at the flow rate of 1.0 mL/min.The detection wavelength was set as 210 nm,and column temperature was 35℃.The sample size was 5μL.The content of total flavonoids was determined by UV-visible spectrophotometry under detection wavelength of 510 nm.RESULTS:The linear range of syringin and total flavonoids were 0.0959-1.1508 mg/mL(r=0.9996)and 0.0722-1.0830 mg/mL(r=0.9999),respectively.RSDs of precision,stability and repeatability tests were all less than 3%(n=6).The average recoveries were 101.74%(RSD=2.36%,n=6)and 99.63%(RSD=2.19%,n=6),respectively.During different harvesting periods,the contents of syringin in root bark,tree bark,leaf of T.angulata collected on Aug.,May and Sept.were the highest,and the contents of total flavonoids in samples collected on Feb.,Dec.and Sept.were the highest.The contents of syringin in different medicinal parts of T.angulata were in descending order as follows as tree bark>root bark>leaf,and the content of syringin was commonly relatively high in tree bark part;the content of total flavonoids in different medicinal parts of T.angulata were in descending order as follows as root bark>tree bark>leaf,and the contents of total flavonoids in three medicinal parts was generally low.The content of total flavonoids in root bark was the highest in Feb.of that year,and the content of syringin in root bark at same month was second only to Aug.of that year;the content of syringin in tree bark was the highest in May,and the content of total flavonoids in tree bark at same month was second only to Oct.and Dec.of that year;the contents of total flavonoids and syringin in leaf were the highest in Sept.of that year.CONCLUSIONS:It is suggested that Feb.is the best time for harvesting root bark,May for tree bark and Sept.for leaf of T.angulata.