不同生产工艺蜂胶中重金属含量分析比较

Analysis and comparison of heavy metal content in propolis with different processing technologies

目的了解不同加工工艺的蜂胶加工品对去除原胶中有害重金属的效果。方法采用微波消解,石墨炉原子吸收光谱法和氢化物发生原子荧光光谱法测定两种蜂胶加工品中的4种有害重金属(铅、镉、总汞、总砷)含量并测定3个浓度的加标回收率。结果 4种元素标准曲线相关系数均在0.999以上,加标回收率乙醇萃取法在83.8%~114.2%,超临界萃取法在72.6%~108.5%之间,采用超临界萃取法加工蜂胶中铅、镉、总砷含量明显低于采用乙醇萃取法加工蜂胶,总汞含量在两种加工工艺中均未检出。结论在有害重金属含量的食品安全性上超临界萃取法的加工工艺优于乙醇萃取法。

Objective To understand the effect of propolis processed products with different processing technologies on removing harmful heavy metals from raw glue. Methods Microwave digestion, graphite furnace atomic absorption spectrometry and hydride generation atomic fluorescence spectrometry were used to determine the contents of four harmful heavy metals(lead, cadmium, total mercury and total arsenic) in 2 propolis processed products and the recovery rates of 3 concentrations were determined. Results The correlation coefficients of the standard curves of the 4 elements were all above 0.999, the recovery rates of standard addition were 83.8%-114.2% by ethanol extraction and 72.6%-108.5% by supercritical fluid extraction. The contents of lead, cadmium and total arsenic in propolis processed by supercritical extraction were significantly lower than those processed by ethanol extraction, and the total mercury content was not detected in both processing technologies. Conclusion Supercritical extraction is better than ethanol extraction in food safety of harmful heavy metal content.