不同碳氮源对糙皮侧耳木质纤维素酶活性的影响

Effects of different carbon and nitrogen sources on lignocellulolytic enzyme activities of Pleurotus ostreatus

以糙皮侧耳Pleurotus ostreatus为材料,研究简单碳氮源及木质素纯品诱导条件对其木质纤维素酶活性的影响。结果表明,不同的碳源培养基和氮源培养基对糙皮侧耳漆酶活性、羧甲基纤维素酶活性和木聚糖酶活性均具有极显著的影响(P<0.001),且对糙皮侧耳菌丝生物量也有极显著的影响(P<0.001)。以蔗糖作主要碳源诱导物时,有利于提高糙皮侧耳漆酶活性;以果糖作主要碳源诱导物时,有利于提高糙皮侧耳羧甲基纤维素酶活性和菌丝生物量的积累;以葡萄糖作主要碳源诱导物时,有利于提高糙皮侧耳木聚糖酶活性。以酵母浸粉作主要氮源诱导物时,有利于提高糙皮侧耳漆酶活性和菌丝生物量的积累;以硝酸钾作为主要氮源诱导物时,有利于提高糙皮侧耳羧甲基纤维素酶活性;以硫酸铵作为主要氮源诱导物时,有利于提高糙皮侧耳木聚糖酶活性。碱性木素的存在,有利于提高糙皮侧耳漆酶活性,但不利于菌丝生物量的积累。与此同时,碱性木素的存在对糙皮侧耳羧甲基纤维素酶和木聚糖酶活性并没有促进作用。

Pleurotus ostreatus was used as the material to study the differences of lignocellulolytic enzyme activities affected by different simple carbon and nitrogen sources and pure lignin.The results showed that the laccase,carboxymethyl cellulase and xylanase activities in P.ostreatus cultivated in the media with different carbon source and nitrogen source was significantly different(P<0.001),and the mycelial biomass obtained was also significantly different(P<0.001).Sucrose used as the main carbon source advantaged the enhancement of laccase activities.Fructose used as the main carbon source was favorable for enhancing carboxymethyl cellulase activities and accumulation of mycelial biomass.Glucose used as the main carbon source was favorable for enhancing of xylanase activities.The enhancement of laccase activities and the accumulation of mycelial biomass were advantaged by yeast extract used as the main nitrogen source.Potassium nitrate used as the main nitrogen source was favorable for enhancement of carboxymethyl cellulase activities.Ammonium sulfate used as the main nitrogen source was favorable for enhancement of xylanase activities.The existence of alkaline lignin was beneficial to the enhancement of laccase activities,but not to the accumulation of mycelial biomass.The presence of alkaline lignin did not promote carboxymethyl cellulase and xylanase activities.