摘要
通过液体振荡-静置两阶段发酵获得灵芝菌丝体,并采用硅胶柱色谱层析、反相柱层析和甲醇重结晶的方法,从中分离得到4个三萜类化合物。根据NMR、MS等波谱数据分析,化合物分别被鉴定为lanosta-7,9(11),24-trien-3α-acetoxy-26-oicacid(1)、灵芝酸R(2)、灵芝酸T(3)和灵芝酸S(4),其中化合物1的核磁信号全归属为首次报道。4个三萜类化合物均具有较好的抑制肿瘤细胞L1210及K562增殖的活性,且化合物1的体外抗肿瘤活性为首次证实,其对肿瘤细胞L1210及K562增殖的半数抑制浓度IC50分别为22.17μmol/L和54.79μmol/L。
Ganoderma lingzhi mycelia were obtained by liquid shaking-static two-stage fermentation method,and four triterpenes were isolated from the mycelia through silica gel column chromatography,reversed-phase column chromatography and methanol recrystallization.According to the analysis of spectral data of NMR and MS,these compounds were identified as lanosta-7,9(11),24-trien-3α-acetoxy-26-oic acid(1),ganoderic acid R(2),ganoderic acid T(3)and ganoderic acid S(4).The nuclear magnetic signals of compound 1 were reported for the first time.All of the four triterpenes showed good inhibitory activity on the proliferation of tumor cells L1210 and K562,and the in vitro antitumor activity of compound 1 was confirmed for the first time.IC50 values of compound 1 against tumor cells L1210 and K562 were 22.17μmol/L and 54.79μmol/L,respectively.
作者
朱晓璐
岳亚文
张劲松
冯杰
唐庆九
冯娜
韩伟
ZHU Xiao-Lu;YUE Ya-Wen;ZHANG Jing-Song;FENG Jie;TANG Qing-Jiu;FENG Na;HAN Wei(Engineering Research Centre of Pharmaceutical Process Chemistry,Ministry of Education,Shanghai Key Laboratory of New Drug Design,School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,National Engineering Research Center of Edible Fungi,Key Laboratory of Edible Fungi Resources and Utilization(South),Ministry of Agriculture of P.R.China,Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2020年第8期1551-1558,共8页
Mycosystema
基金
上海市科技兴农重点攻关项目[沪农科创字(2018)第1-1号]。
关键词
灵芝
两阶段发酵
三萜类
核磁归属
增殖抑制
Ganoderma lingzhi
two-stage fermentation
triterpenes
NMR attribution
proliferation inhibition
