长裙竹荪抑菌活性物质的分离纯化及其抑菌效果

Separation and purification of antibacterial component from Dictyophora indusiata and determination of its antibacterial effects

长裙竹荪Dictyophora indusiata是珍贵的食药用真菌,具有很强的抑菌作用,在天然防腐剂开发方面具有广阔的应用前景。本研究以长裙竹荪的抑菌活性为指标,通过萃取、3次不同流动相的硅胶柱层析、1次反相柱层析和薄层层析法对竹荪提取物进行分离纯化,得到一个抗菌活性强的单体化合物。根据核磁共振波谱等数据分析,推断该化合物为间苯三酚。以巨大芽孢杆菌和肠炎沙门氏菌为供试菌,用平板打孔法及原位抑菌法测定该化合物的抑菌效果,结果表明:该化合物对这两种菌有很强的抑制作用,半抑制浓度分别为83.06μg/m L和51.58μg/m L。本研究首次从长裙竹荪中获得具有抗菌活性的单体化合物间苯三酚,为竹荪天然抗菌物质的开发提供理论依据。

Dictyophora indusiata,a kind of precious edible and medicinal fungus,has strong antimicrobial activity,and is expected to be used as natural preservative for food preservation.In this study,an antimicrobial component from D.indusiata was isolated and purified by solvent extraction,three times silica gel column chromatography with different mobile phase,one time reversed phase column chromatography and thin layer chromatography.This compound was identified as m-trihydroxybenzene by nuclear magnetic resonance spectroscopy(NMR)analysis.Using Bacillus magaterium and Salmonella enteritidis as test bacteria,the antibacterial effect of this compound was determined by plate perforation,in situ bacteriostasis and microscopic observation.The results showed that the compound had a strong inhibitory effect on the two bacteria,with semi-inhibitory concentration of 83.06 g/mL and 51.58 g/mL,respectively.Individual compound m-trihydroxybenzene with antibacterial activity is firstly obtained from D.indusiata and hopefully developable as natural antibacterial substances.