温胃舒胶囊通过抑制核因子κB(NF-κB)通路减轻慢性胃炎大鼠胃黏膜损伤

Wenweishu Capsule alleviates gastric mucosal lesion in rats with chronic gastritis by inhibiting NF-κB pathway

目的探讨温胃舒胶囊对慢性胃炎模型大鼠核因子κB(NF-κB)相关蛋白表达的影响。方法采用脱氧胆酸钠、氨水、乙醇和饥饱失常法建立慢性胃炎Wistar大鼠模型。分为空白组、模型组、维酶素片组、温胃舒胶囊高、中、低剂量组,空白组、模型组以生理盐水2 mL/d、其余各组分别以0.3 g/kg维酶素及(0.76、0.38、0.19)g/kg温胃舒胶囊灌胃,处理4周。肉眼观察及HE染色评价胃组织病变情况,ELISA检测大鼠血清肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)水平,免疫荧光染色观察胃组织核因子κBp65(NF-κBp65)、NF-κB抑制蛋白α(IκBα)的表达,Simple Western法检测胃组织NF-κBp65、IκBα、环加氧酶2(COX2)蛋白水平。结果与空白组比较,模型组大鼠胃黏膜变薄、皱襞消失或变浅,胃黏膜炎性细胞浸润明显、腺体紊乱,血清炎性因子及胃组织NF-κBp65、IκBα、COX2蛋白水平显著升高;与模型组比较,维酶素组、温胃舒胶囊高、中剂量组大鼠胃腔体征减轻,组织病变改善,血清中TNF-α和IL-6水平降低,胃组织中NF-κBp65、IκBα、COX2蛋白表达降低。结论温胃舒胶囊通过抑制胃组织NF-κB通路,降低血清炎症因子水平,减轻慢性胃炎大鼠胃黏膜损伤。

Objective To investigate the effect of Wenweishu Capsule on the expression of nuclear factor kappa B(NF-κB)-related proteins in chronic gastritis model rats.Methods Wistar rat models of chronic gastritis were constructed by alternant administrations of sodium deoxycholate,ammonia,alcohol solution and the hunger disorder method.The rats were randomly divided into control group,model group,vatacoenayme group,high-,middle-and low-dose Wenweishu Capsule groups.The control group and model group were treated with normal saline(2 mL/d).The other groups were separately treated with 0.3 g/kg vatacoenayme and 0.76,0.38,0.19 g/kg Wenweishu Capsule for 4 weeks.Naked eye observation and HE staining were used to evaluate the pathological changes of gastric tissue.ELISA was performed to measure the levels of tumor necrosis factorα(TNF-α)and interleukin 6(IL-6)in the serum.Immunofluorescence staining was employed to observe the expression of NF-κBp65,inhibitor of NF-κBα(IκBα)in the gastric tissue.Simple Western was utilized to detect the protein levels of NF-κBp65,IκBαand COX2 in the gastric tissue.Results Compared with the control group,the model group was found with thinner gastric mucosa,disappeared or shallower folds,obviously infiltrated mucosa inflammatory cells,disordered glands,and significantly increased levels of serum inflammatory cytokines and NF-κBp65,IκBαand COX2 proteins in the gastric tissue.Compared with the model group,the vatacoenayme group,high-and middle-dose Wenweishu Capsule groups showed the alleviated gastric cavity signs,improved histopathological changes,reduced levels of TNF-αand IL-6 in the serum,and decreased expression of NF-κBp65,IκBαand COX2 proteins in the gastric tissue.ConclusionWenweishu Capsule can reduce the levels of serum inflammatory factors by inhibiting NF-κB pathway in the gastric tissue,so as to alleviate the injury of gastric mucosa in rats with chronic gastritis.